Anti-Tissue Factor抗体(ab104513)


  • 产品名称Anti-Tissue Factor抗体
    参阅全部 Tissue Factor 一抗
  • 描述
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 33 - 295 of Human Tissue Factor (BC011029).

  • 阳性对照
    • WB: Human fetal kidney, brain, skin and lung lysates IHC-P: Human fetal kidney tissue



Our Abpromise guarantee covers the use of ab104513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/500.


Anti-Tissue Factor antibody 图像

  • All lanes : Anti-Tissue Factor antibody (ab104513) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal skin lysate
    Lane 4 : Human fetal lung lysate

    Predicted band size : 33 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab104513, at a 1/100 dilution, staining Tissue Factor in formalin-fixed, paraffin-embedded Human fetal kidney tissue, showing membrane staining.

Anti-Tissue Factor antibody (ab104513)参考文献

This product has been referenced in:
  • Guiraut C  et al. Perinatal Arterial Ischemic Stroke Is Associated to Materno-Fetal Immune Activation and Intracranial Arteritis. Int J Mol Sci 17:N/A (2016). IHC-P ; Rat . Read more (PubMed: 27898024) »
  • Mizugishi K  et al. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. J Biol Chem 290:2053-68 (2015). IHC-P ; Mouse . Read more (PubMed: 25505239) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - other (aortic vascular smooth muscle cells)
Specification aortic vascular smooth muscle cells
Treatment Indoxy sulfate 0.25-100 ug/ml
Blocking step Milk as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Dr. Moshe Shashar

Verified customer

提交于 Jul 18 2014

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

Read More

Thanks for the additional information.

I have initiated the request for a replacement of one vial of ab104513. Please contact me again if you have any further questions.

Thank you for submitting your completed questionnaire.

It does appear that this antibody is not working as expected. If you have received this antibody within the past 6 months or so I would be happy to offer a replacement.

In your ...

Read More
Application Flow Cytometry
Sample Rat Cell (Plasma Microparticles)
Specification Plasma Microparticles
Preparation Cell harvesting/tissue preparation method: Centrifuge whole blood 1,500xg 15 min, then centrifuge the supernatant again at 13,000xg 2 min, and retain supernatant
Sample buffer: Annexin Binding Buffer
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy MP samples without label, and sample with secondary antibody only

Abcam user community

Verified customer

提交于 Feb 23 2012

I am very pleased to hear you would like to accept our offer and test ab104513 in flow cytometry using rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forflow cyto...

Read More