Anti-Tissue Factor抗体[2K1] (ab17375)

概述

  • 产品名称Anti-Tissue Factor抗体[2K1]
    参阅全部 Tissue Factor 一抗
  • 描述
    小鼠单克隆抗体[2K1] to组织Factor
  • 经测试应用适用于: WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human tissue factor apoprotein (47kD)

  • 表位Epitope is within the extracellular domain. Specific for epitope locus I.

性能

应用

Our Abpromise guarantee covers the use of ab17375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/200.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • 序列相似性Belongs to the tissue factor family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Anti-Tissue Factor antibody [2K1] 图像

  • ab17375 staining Tissue Factor by Immunocytochemistry/ Immunofluorescence in mouse BxPC-3 pancreatic adenocarcinoma cells. Cells were fixed with paraformaldehyde and blocking with 1% BSA and 1% goat serum was performed for 1 hour at 240C. Samples were incubated with primary antibody (1/200: in 1% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody.  

    See Abreview

  • ab17375 staining Tissue Factor in human nasopharynx tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0, 100°C for 20 minutes. Samples were then incubated with the primary antibody at a 1/200 dilution for 20 minutes at 25°C. An HRP-conjugated goat anti-mouse/rabbit polyclonal was used undiluted as the secondary antibody.

    See Abreview

Anti-Tissue Factor antibody [2K1] (ab17375)参考文献

This product has been referenced in:
  • Cugno M  et al. Enhanced tissue factor expression by blood eosinophils from patients with hypereosinophilia: a possible link with thrombosis. PLoS One 9:e111862 (2014). WB ; Human . Read more (PubMed: 25375118) »
  • Liu X  et al. Elevated immunoreactivity to tissue factor and its association with dysmenorrhea severity and the amount of menses in adenomyosis. Hum Reprod 26:337-45 (2011). IHC-P ; Human . Read more (PubMed: 21115504) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. I would like to reassure you the quality of these antibodies may not have been affected by remaining at 4C during two weeks.

We recently undertook an in-depth study examining storage conditions and antibody perfo...

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Thank you for your inquiry. Please see the answer to your questions below: 1.) I can confirm that ab17375 (Anti-Tissue Factor antibody [2K1]) is derived from supernatant from hybridoma cells whereas ab35807 (Anti-Tissue Factor antibody ...

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I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued three free of charge replacements. To check the status of the order please contact our Customer Service team...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (nasopharynx)
Specification nasopharynx
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0, 20 min, 100C
Username

Abcam user community

Verified customer

提交于 Dec 17 2010

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (CB17/SCID BxPC-3 xenograft)
Specification CB17/SCID BxPC-3 xenograft
Fixative Paraformaldehyde
Permeabilization No
Blocking step 1% BSA, 1% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Dr. Jennifer Dembinski

Verified customer

提交于 Mar 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human umbilical vein endothelial cells)
Loading amount 30 µg
Specification Human umbilical vein endothelial cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"