Mouse, Rat, Cow, Dog
Synthetic peptide corresponding to Human TIS11B aa 46-95 (N terminal). The immunogen was taken from within these 50aa's
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.125 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent dilution. PubMed: 20691152
Probable regulatory protein involved in regulating the response to growth factors.
Contains 2 C3H1-type zinc fingers.
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
Information by UniProt
Butyrate response factor 1 antibody
Western blot - Anti-TIS11B antibody (ab42473)
Anti-TIS11B antibody (ab42473) at 0.125 µg/ml (5% skimmed milk / PBS) + 10µg Human placenta whole cell lysate
HRP conjugated anti-rabbit at 1/50000 dilution
Predicted band size:
Observed band size:
30 kDa (
why is the actual band size different from the predicted?
Gel concentration 12%.
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