概述

  • 产品名称Anti-TIS11B抗体
    参阅全部 TIS11B 一抗
  • 描述
    兔多克隆抗体to TIS11B
  • 经测试应用适用于: ELISA, WB, EMSAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human TIS11B aa 46-95 (N terminal). The immunogen was taken from within these 50aa's
    Sequence:

    GGGFPRRHSVTLPSSKFHQNQLLSSLKGEPAPALSSRDSRFRDRSFSEGG


    Database link: Q07352

  • 阳性对照
    • Human placenta lysate.

性能

应用

Our Abpromise guarantee covers the use of ab42473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
EMSA Use at an assay dependent dilution. PubMed: 20691152

靶标

Anti-TIS11B antibody 图像

  • Anti-TIS11B antibody (ab42473) at 0.125 µg/ml (5% skimmed milk / PBS) + 10µg Human placenta whole cell lysate

    Secondary
    HRP conjugated anti-rabbit at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%.

Anti-TIS11B antibody (ab42473)参考文献

This product has been referenced in:
  • Kim CW  et al. Ectopic over-expression of tristetraprolin in human cancer cells promotes biogenesis of let-7 by down-regulation of Lin28. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 22210895) »
  • Kim CW  et al. Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR of cIAP2 mRNA. Biochem Biophys Res Commun 400:46-52 (2010). EMSA ; Human . Read more (PubMed: 20691152) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab42473.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"