The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Use at 2-5 µg/mg of lysate.
May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.
Belongs to the TIP41 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling TIPRL with ab70795 at 1/200 (1µg/ml). Detection: DAB.
Western blot - TIPRL antibody (ab70795)
All lanes : Anti-TIPRL antibody (ab70795) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml Lane 2 : HeLa whole cell lysate at 15 µg/ml Lane 3 : HeLa whole cell lysate at 5 µg/ml Lane 4 : 293T whole cell lysate at 50 µg/ml Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 32 kDa Observed band size: 32 kDa
Exposure time: 3 seconds
Immunoprecipitation - TIPRL antibody (ab70795)
Detection of TIPRL by Western Blot of Immunoprecipitate.
ab70795 at 1µg/ml staining TIPRL in HeLa whole cell lysate immunoprecipitated using ab70795 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
Tirosh O et al. The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions. PLoS Pathog11:e1005288 (2015).
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