概述

  • 产品名称Anti-TIPRL抗体
    参阅全部 TIPRL 一抗
  • 描述
    兔多克隆抗体to TIPRL
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 225 and the C-terminus (residue 272) of human TIPRL (NP_690866.1)

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab70795 was affinity purified using an epitope specific to TIPRL immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.
  • 序列相似性Belongs to the TIP41 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1810011K17Rik antibody
    • dJ69E11.3 antibody
    • MGC3794 antibody
    • Putative MAPK activating protein PM10 antibody
    • Putative MAPK-activating protein PM10 antibody
    • RP1-69E11.1 antibody
    • Tip antibody
    • TIP41 antibody
    • TIP41-like protein antibody
    • tiprl antibody
    • TIPRL_HUMAN antibody
    • TOR Signaling Pathway Regulator like antibody
    • Type 2A-interacting protein antibody
    see all

Anti-TIPRL antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling TIPRL with ab70795 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TIPRL antibody (ab70795) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml
    Lane 4 : 293T whole cell lysate at 50 µg/ml
    Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 32 kDa


    Exposure time : 3 seconds
  • Detection of TIPRL by Western Blot of Immunoprecipitate.
    ab70795 at 1µg/ml staining TIPRL in HeLa whole cell lysate immunoprecipitated using ab70795 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-TIPRL antibody (ab70795)参考文献

ab70795 has not yet been referenced specifically in any publications.

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