概述

  • 产品名称Anti-TIP49A抗体
    参阅全部 TIP49A 一抗
  • 描述
    兔多克隆抗体to TIP49A
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Chimpanzee, Zebrafish, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human TIP49A.

  • 阳性对照
    • This antibody gave a positive signal in Human Testis tissue lysate as well as the following whole cell lysates: HeLa; Jurkat; HepG2. This antibody gave a positive result in IHC in the following FFPE tissue: Humannormal testis. This antibody gave a positive result in ICC/IF in the following formaldehyde fixed cell line: MCF-7

性能

应用

Our Abpromise guarantee covers the use of ab109330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
    May be able to bind plasminogen at cell surface and enhance plasminogen activation.
    Essential for cell proliferation.
  • 组织特异性Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
  • 序列相似性Belongs to the ruvB family.
  • 结构域Binding to MYC is dependent on a Myc domain essential for oncogenic activity.
  • 细胞定位Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 49 kDa TATA box binding protein interacting protein antibody
    • 49 kDa TATA box-binding protein-interacting protein antibody
    • 49 kDa TBP interacting protein antibody
    • 49 kDa TBP-interacting protein antibody
    • 54 kDa erythrocyte cytosolic protein antibody
    • ECP-54 antibody
    • ECP54 antibody
    • ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD antibody
    • INO80 complex subunit H antibody
    • NMP 238 antibody
    • Nuclear matrix protein 238 antibody
    • Pontin 52 antibody
    • PONTIN antibody
    • RuvB like 1 antibody
    • RuvB-like 1 antibody
    • RUVB1_HUMAN antibody
    • RUVBL1 antibody
    • RVB1 antibody
    • TAP54 alpha antibody
    • TAP54-alpha antibody
    • TIP49 antibody
    • TIP49a antibody
    • TIP60 associated protein 54 alpha antibody
    • TIP60-associated protein 54-alpha antibody
    see all

Anti-TIP49A antibody 图像

  • ab109330 stained MCF-7 cells. The cells were 4% PFA fixed for 10 minutes at Room Temperature and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109330 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-TIP49A antibody (ab109330) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 36 kDa,42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • IHC image of TIP49A staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109330, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-TIP49A antibody (ab109330)参考文献

ab109330 has not yet been referenced specifically in any publications.

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