Anti-TIP49A抗体[2943C1a] (ab51500)

概述

• 产品名称
  Anti-TIP49A抗体[2943C1a]
  参阅全部 TIP49A 一抗
• 描述
  小鼠单克隆抗体[2943C1a] to TIP49A
• 经测试应用
  适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details
• 种属反应性
  与反应: Mouse, Human
  
• 免疫原

  Recombinant fragment, internal sequence. (Human)

• 阳性对照
  • HT-1080 whole cell lysate, HeLa cells.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• 存储溶液
  Preservative: 0.05% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• 纯度
  Protein G purified
• 纯化说明
  ab51500 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• 克隆
  单克隆
• 克隆编号
  2943C1a
• 同种型
  IgG2b
• 研究领域
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

相关产品

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

应用

靶标

• 功能
  Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
  May be able to bind plasminogen at cell surface and enhance plasminogen activation.
  Essential for cell proliferation.
• 组织特异性
  Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
• 序列相似性
  Belongs to the ruvB family.
• 结构域
  Binding to MYC is dependent on a Myc domain essential for oncogenic activity.
• 细胞定位
  Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.

• Target information above from: UniProt accession Q9Y265 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 8607 Human
  • Entrez Gene: 56505 Mouse
  • Omim: 603449 Human
  • SwissProt: Q9Y265 Human
  • SwissProt: P60122 Mouse
  • Unigene: 272822 Human
  • Unigene: 42195 Mouse
• 别名
  • 49 kDa TATA box binding protein interacting protein antibody
  • 49 kDa TATA box-binding protein-interacting protein antibody
  • 49 kDa TBP interacting protein antibody

  • 49 kDa TBP-interacting protein antibody
  • 54 kDa erythrocyte cytosolic protein antibody
  • ECP-54 antibody
  • ECP54 antibody
  • ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD antibody
  • INO80 complex subunit H antibody
  • NMP 238 antibody
  • Nuclear matrix protein 238 antibody
  • Pontin 52 antibody
  • PONTIN antibody
  • RuvB like 1 antibody
  • RuvB-like 1 antibody
  • RUVB1_HUMAN antibody
  • RUVBL1 antibody
  • RVB1 antibody
  • TAP54 alpha antibody
  • TAP54-alpha antibody
  • TIP49 antibody
  • TIP49a antibody
  • TIP60 associated protein 54 alpha antibody
  • TIP60-associated protein 54-alpha antibody

  see all

实验方案

• Flow cytometry protocols
• Immunohistochemistry protocols
• Immunocytochemistry & immunofluorescence protocols
• Western blot protocols