概述

  • 产品名称Anti-TIP49A抗体[2943C1a]
    参阅全部 TIP49A 一抗
  • 描述
    小鼠单克隆抗体[2943C1a] to TIP49A
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, internal sequence. (Human)

  • 阳性对照
    • HT-1080 whole cell lysate, HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab51500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 0.5 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
    May be able to bind plasminogen at cell surface and enhance plasminogen activation.
    Essential for cell proliferation.
  • 组织特异性Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
  • 序列相似性Belongs to the ruvB family.
  • 结构域Binding to MYC is dependent on a Myc domain essential for oncogenic activity.
  • 细胞定位Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 49 kDa TATA box binding protein interacting protein antibody
    • 49 kDa TATA box-binding protein-interacting protein antibody
    • 49 kDa TBP interacting protein antibody
    • 49 kDa TBP-interacting protein antibody
    • 54 kDa erythrocyte cytosolic protein antibody
    • ECP-54 antibody
    • ECP54 antibody
    • ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD antibody
    • INO80 complex subunit H antibody
    • NMP 238 antibody
    • Nuclear matrix protein 238 antibody
    • Pontin 52 antibody
    • PONTIN antibody
    • RuvB like 1 antibody
    • RuvB-like 1 antibody
    • RUVB1_HUMAN antibody
    • RUVBL1 antibody
    • RVB1 antibody
    • TAP54 alpha antibody
    • TAP54-alpha antibody
    • TIP49 antibody
    • TIP49a antibody
    • TIP60 associated protein 54 alpha antibody
    • TIP60-associated protein 54-alpha antibody
    see all

Anti-TIP49A antibody [2943C1a] 图像

  • Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution + HT-1080 whole cell lysate at 50 µg

    Secondary
    Anti Mouse IgG at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • Flow cytometry using ab51500 at 0.5ug using HeLa cells. Secondary used was an AlexaFluor® 488 conjugated Goat Anti-mouse IgG (0.25 µg). White peak shows an isotype control, while the red peak shows ab51500.
  • All lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1 µg/ml (in 3% BSA + PBS-Tween 0.1% for 12 hours at 4°C)

    Lane 1 : HeLa cell lysate + control siRNA sequence
    Lane 2 : HeLa cell lysate + control siRNA sequence
    Lane 3 : HeLa cell lysate suppressed by siRNA (1) sequence to pontin
    Lane 4 : HeLa cell lysate suppressed by siRNA (2) sequence to pontin
    Lane 5 : Cell lysate of 293T cells
    Lane 6 : Cell lysate of HeLa cells
    Lane 7 : Cell lysate of U251 cells
    Lane 8 : Cell lysate of Jurkat cells
    Lane 9 : Cell lysate of U2OS cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC image of ab51500 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51500, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution

    Lane 1 : Whole cell lysate prepared from 9L cells
    Lane 2 : Whole cell lysate prepared from PC12 cells
    Lane 3 : Whole cell lysate prepared from RIE-1 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-mouse polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-TIP49A antibody [2943C1a] (ab51500)参考文献

This product has been referenced in:
  • Queval R  et al. Reptin and Pontin oligomerization and activity are modulated through histone H3 N-terminal tail interaction. J Biol Chem 289:33999-4012 (2014). Read more (PubMed: 25336637) »
  • Gnatovskiy L  et al. The human RVB complex is required for efficient transcription of type I interferon-stimulated genes. Mol Cell Biol 33:3817-25 (2013). Human . Read more (PubMed: 23878400) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (Rat cell lines)
Loading amount 20 µg
Specification Rat cell lines
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 2°C
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Abcam user community

Verified customer

提交于 Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF, F9, GC-1, NIH-3T3)
Loading amount 20 µg
Specification MEF, F9, GC-1, NIH-3T3
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Jul 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 20 µg
Specification Hela
Treatment siRNA to Pontin
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"