重组Anti-TIP30抗体[EPR13179] (ab177961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13179] to TIP30
- Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-TIP30抗体[EPR13179]
参阅全部 TIP30 一抗 -
描述
兔单克隆抗体[EPR13179] to TIP30 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human TIP30 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BUP3 -
阳性对照
- HeLa, A431, and fetal liver lysates; Human pancreas and placenta tissues; A431 cells; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13179 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177961于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP |
1/10 - 1/100.
|
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
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WB |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
|
|
ICC/IF |
1/50 - 1/100.
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect. -
组织特异性
Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas. -
结构域
Unique C-terminus confers high proteasome-dependent instability to isoform 2. -
细胞定位
Cytoplasm. Nucleus envelope. - Information by UniProt
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数据库链接
- Entrez Gene: 10553 Human
- Entrez Gene: 53415 Mouse
- Entrez Gene: 292935 Rat
- Omim: 605628 Human
- SwissProt: Q9BUP3 Human
- SwissProt: Q9Z2G9 Mouse
- Unigene: 90753 Human
- Unigene: 20801 Mouse
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别名
- 30 kDa HIV 1 TAT interacting protein antibody
- 30 kDa HIV-1 TAT-interacting protein antibody
- 30 kDa HIV1 TAT interacting protein antibody
see all
图片
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All lanes : Anti-TIP30 antibody [EPR13179] (ab177961) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling TIP30 using ab177961 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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Immunofluorescence analysis of A431 cells labeling TIP30 using ab177961 at 1/50 dilution.
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Immunohistochemical analysis of Human placenta tissue labeling TIP30 using ab177961 at 1/50 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemical analysis of Human pancreas tissue labeling TIP30 using ab177961 at 1/50 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab177961 at 1/10 dilution to immunoprecipitate TIP30, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab177961 被引用在 4 文献中.
- Lin X et al. Long Non-Coding RNA NONHSAT076754 Promotes Invasion and Metastasis in Epithelial Ovarian Cancer. J Cancer 10:1930-1940 (2019). PubMed: 31205552
- Grund A et al. TIP30 counteracts cardiac hypertrophy and failure by inhibiting translational elongation. EMBO Mol Med 11:e10018 (2019). PubMed: 31468715
- Yang Q et al. Metformin Enhances the Effect of Regorafenib and Inhibits Recurrence and Metastasis of Hepatic Carcinoma After Liver Resection via Regulating Expression of Hypoxia Inducible Factors 2a (HIF-2a) and 30 kDa HIV Tat-Interacting Protein (TIP30). Med Sci Monit 24:2225-2234 (2018). PubMed: 29654226
- You A et al. Metformin sensitizes sorafenib to inhibit postoperative recurrence and metastasis of hepatocellular carcinoma in orthotopic mouse models. J Hematol Oncol 9:20 (2016). WB ; Human . PubMed: 26957312