Anti-CAND1抗体(ab101987)
Key features and details
- Rabbit polyclonal to CAND1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CAND1抗体
参阅全部 CAND1 一抗 -
描述
兔多克隆抗体to CAND1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to Human CAND1 aa 1180-1230 (internal sequence).
Database link: NP_060918.2 -
阳性对照
- 293T, NIH3T3 and HeLa cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101987于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 136 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Enhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins. -
序列相似性
Belongs to the CAND family.
Contains 27 HEAT repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 452060 Chimpanzee
- Entrez Gene: 538086 Cow
- Entrez Gene: 55832 Human
- Entrez Gene: 71902 Mouse
- Entrez Gene: 100173509 Orangutan
- Entrez Gene: 117152 Rat
- Omim: 607727 Human
- SwissProt: A7MBJ5 Cow
see all -
别名
- CAND 1 antibody
- Cand1 antibody
- CAND1_HUMAN antibody
see all
图片
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All lanes : Anti-CAND1 antibody (ab101987) at 0.1 µg
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Predicted band size: 136 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse hybridoma tumor (right) tissues labelling CAND1 with ab101987 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
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Detection of CAND1 by Western blot of Immunprecipitate.
ab101987, at 1 µg/ml, staining CAND1 in HeLa whole cell lysate immunoprecipitated using ab101987 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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