产品名称 Anti-TIMP4抗体 参阅全部 TIMP4 一抗
特异性 ab58425 detects endogenous levels of total TIMP4 protein.
IHC-Fr, ELISA, IHC-P, WB more details
Mouse, Rat, Human
Synthetic peptide derived from C terminal residues of human TIMP4
IHC-P: Human lung carcinoma tissue.
WB: NIH3T3 cell extracts.
存放说明 Store at -20°C. Stable for 12 months at -20°C
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab58425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23525608
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
功能 Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
组织特异性 Abundant in heart and present at low levels in many other tissues.
序列相似性 Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain.
Information by UniProt
Metalloproteinase inhibitor 4 antibody
TIMP 4 antibody
TIMP metallopeptidase inhibitor 4 antibody
Anti-TIMP4 antibody 图像
Western blot - TIMP4 antibody (ab58425)
All lanes : Anti-TIMP4 antibody (ab58425) at 1/500 dilution Lane 1 : NIH3T3 cell extract Lane 2 : NIH3T3 cell extract with immunising peptide Predicted band size : 26 kDa Observed band size : 26 kDa
Immunohistochemistry (Paraffin-embedded sections) - TIMP4 antibody (ab58425)
ab58425, at 1/50 dilution, staining TIMP4 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunising peptide.
Anti-TIMP4 antibody (ab58425)参考文献
This product has been referenced in:
et al. Hyperhomocysteinemia during aortic aneurysm, a plausible role of epigenetics. Int J Physiol Pathophysiol Pharmacol 5:32-42 (2013).
Read more (PubMed: 23525608) »
et al. Maternal hypoxia alters matrix metalloproteinase expression patterns and causes cardiac remodeling in fetal and neonatal rats. Am J Physiol Heart Circ Physiol 301:H2113-21 (2011).
Read more (PubMed: 21856922) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"