概述

  • 产品名称Anti-TIMP3抗体[136-13H4]
    参阅全部 TIMP3 一抗
  • 描述
    小鼠单克隆抗体[136-13H4] to TIMP3
  • 特异性Recognizes the ~27 kDa glycosylated and the ~24 kDa non-glycosylated forms of TIMP3. Does not cross-react with TIMP1 or TIMP2.
  • 经测试应用适用于: IHC-P, WB, IHC-Frmore details
    不适用于: ICC/IF or IP
  • 种属反应性
    与反应: Rabbit, Human, Baboon
    预测可用于: Mouse, Horse, Xenopus laevis, Cynomolgus Monkey, Macaque Monkey不与反应: Rat, Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide:

    SNFGYPGYQSKHYACIRQK

    , corresponding to amino acids 170-188 of Human TIMP3

  • 阳性对照
    • 293 or Caco-2 cells Unfixed eye tissue

性能

应用

Our Abpromise guarantee covers the use of ab58804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-Fr Use a concentration of 2 - 10 µg/ml.
  • 应用说明Is unsuitable for ICC/IF or IP.
  • 靶标

    • 功能Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
    • 疾病相关Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
    • 序列相似性Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HSMRK222 antibody
      • K222 antibody
      • K222TA2 antibody
      • Metalloproteinase inhibitor 3 antibody
      • MIG 5 protein antibody
      • MIG5 protein antibody
      • Protein MIG 5 antibody
      • Protein MIG-5 antibody
      • SFD antibody
      • Sorsby fundus dystrophy pseudoinflammatory antibody
      • TIMP 3 antibody
      • TIMP metallopeptidase inhibitor 3 antibody
      • TIMP-3 antibody
      • TIMP3 antibody
      • TIMP3_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 3 antibody
      • Tissue inhibitor of metalloproteinases 3 antibody
      • Tissue inhibitor of metalloproteinases3 antibody
      see all

    Anti-TIMP3 antibody [136-13H4] 图像

    • ab58804 (1µg/ml) staining TIMP3 in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic/secreted staining of syncytiotrophoblasts.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    Anti-TIMP3 antibody [136-13H4] (ab58804)参考文献

    This product has been referenced in:
    • Rutnam ZJ  et al. The pseudogene TUSC2P promotes TUSC2 function by binding multiple microRNAs. Nat Commun 5:2914 (2014). WB ; Mouse . Read more (PubMed: 24394498) »
    • Yang X  et al. Both mature miR-17-5p and passenger strand miR-17-3p target TIMP3 and induce prostate tumor growth and invasion. Nucleic Acids Res 41:9688-704 (2013). Read more (PubMed: 23990326) »

    See all 3 Publications for this product

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