Anti-TIMP2抗体(ab64040)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to TIMP2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human TIMP2.

    (Peptide available as ab86642.)

  • 阳性对照
    • This antibody gave a positive signal in Rat Skeletal Muscle Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab64040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

靶标

  • 功能
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • 序列相似性
    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • 翻译后修饰
    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSC 21K antibody
    • CSC-21K antibody
    • CSC21K antibody
    • Metalloproteinase inhibitor 2 antibody
    • Metalloproteinase inhibitor 2 precursor antibody
    • TIMP 2 antibody
    • TIMP metallopeptidase inhibitor 2 antibody
    • TIMP-2 antibody
    • TIMP2 antibody
    • TIMP2_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 2 antibody
    • Tissue inhibitor of metalloproteinases 2 antibody
    see all

Anti-TIMP2 antibody 图像

  • Anti-TIMP2 antibody (ab64040) at 1 µg/ml + Skeletal Muscle (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-TIMP2 antibody (ab64040)参考文献

ab64040 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Airway epithelial cells)
Specification
Airway epithelial cells
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
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提交于 Sep 07 2012

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