概述

  • 产品名称
  • 描述
    兔多克隆抗体to TIMP2
  • 特异性
    ab53730 detects endogenous levels of total TIMP2 protein.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from human TIMP2.

  • 阳性对照
    • A549 cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab53730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 24 kDa).
ELISA 1/40000.

靶标

  • 功能
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • 序列相似性
    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • 翻译后修饰
    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSC 21K antibody
    • CSC-21K antibody
    • CSC21K antibody
    • Metalloproteinase inhibitor 2 antibody
    • Metalloproteinase inhibitor 2 precursor antibody
    • TIMP 2 antibody
    • TIMP metallopeptidase inhibitor 2 antibody
    • TIMP-2 antibody
    • TIMP2 antibody
    • TIMP2_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 2 antibody
    • Tissue inhibitor of metalloproteinases 2 antibody
    see all

图片

  • All lanes : Anti-TIMP2 antibody (ab53730) at 1/500 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with immunising peptide


    Predicted band size : 24 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Smith AC  et al. Synaptic plasticity mediating cocaine relapse requires matrix metalloproteinases. Nat Neurosci 17:1655-7 (2014). IHC ; Mouse . Read more (PubMed: 25326689) »
  • Fortin CF  et al. MT6-MMP is present in lipid rafts and faces inward in living human PMNs but translocates to the cell surface during neutrophil apoptosis. Int Immunol : (2010). Read more (PubMed: 20501611) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your patience.
The specific immunogen information is considered proprietary, but ab53730 binds to the NTR domain of the protein. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting us.
While the immunogen sequences for our antibodies are considered proprietary, we do provide general information about the immunogen range on our datasheets:
ab38972: Synthetic peptide based on the Human sequence. T...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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