重组
RabMAb

Anti-TIMM50抗体[EPR5785] (ab109436)

概述

  • 产品名称
    Anti-TIMM50抗体[EPR5785]
    参阅全部 TIMM50 一抗
  • 描述
    兔单克隆抗体[EPR5785] to TIMM50
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human TIMM50

  • 阳性对照
    • 293T, SH-SY5Y, HepG2, and HeLa cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能
      Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
    • 组织特异性
      Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
    • 序列相似性
      Belongs to the TIM50 family.
      Contains 1 FCP1 homology domain.
    • 结构域
      The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
    • 细胞定位
      Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Homolog of yeast Tim50 antibody
      • Import inner membrane translocase subunit TIM 50 antibody
      • Import inner membrane translocase subunit TIM50 antibody
      • Import inner membrane translocase subunit TIM50 mitochondrial antibody
      • MGC102733 antibody
      • Mitochondrial import inner membrane translocase subunit TIM50 antibody
      • TIM 50 antibody
      • TIM 50L antibody
      • TIM50 antibody
      • Tim50 like protein antibody
      • TIM50_HUMAN antibody
      • TIM50L antibody
      • TIMM 50 antibody
      • TIMM50 antibody
      • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
      • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
      • Translocase of inner mitochondrial membrane 50 homolog antibody
      • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
      see all

    Anti-TIMM50 antibody [EPR5785] 图像

    • All lanes : Anti-TIMM50 antibody [EPR5785] (ab109436) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-TIMM50 antibody [EPR5785] (ab109436)参考文献

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application
    Western blot
    Sample
    Mouse Cell lysate - other (Homogenate of mouse brains, and mitochondria from)
    Gel Running Conditions
    Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol,10 min at 55C denaturation.)
    Loading amount
    20 µg
    Specification
    Homogenate of mouse brains, and mitochondria from
    Blocking step
    SEA Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 10 2017

    Thank you very much for your call yesterday.

    I've heard back from the lab, and the concentration of this lot is 0.932 mg/mL. The hybridoma cells are grown in serum-free media, so the supernatant does not contain any media.

    I hope th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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