RabMAb

Anti-TIMM50抗体[EPR5784] (ab109527)

概述

  • 产品名称Anti-TIMM50抗体[EPR5784]
    参阅全部 TIMM50 一抗
  • 描述
    兔单克隆抗体[EPR5784] to TIMM50
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a region within Human TIMM50.

  • 阳性对照
    • 293T, SH SY5Y, HepG2 and HeLa cell lysates Human kidney tissue, human liver tissue (IHC-P)
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
    • 组织特异性Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
    • 序列相似性Belongs to the TIM50 family.
      Contains 1 FCP1 homology domain.
    • 结构域The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
    • 细胞定位Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Homolog of yeast Tim50 antibody
      • Import inner membrane translocase subunit TIM 50 antibody
      • Import inner membrane translocase subunit TIM50 antibody
      • Import inner membrane translocase subunit TIM50 mitochondrial antibody
      • MGC102733 antibody
      • Mitochondrial import inner membrane translocase subunit TIM50 antibody
      • TIM 50 antibody
      • TIM 50L antibody
      • TIM50 antibody
      • Tim50 like protein antibody
      • TIM50_HUMAN antibody
      • TIM50L antibody
      • TIMM 50 antibody
      • TIMM50 antibody
      • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
      • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
      • Translocase of inner mitochondrial membrane 50 homolog antibody
      • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
      see all

    Anti-TIMM50 antibody [EPR5784] 图像

    • All lanes : Anti-TIMM50 antibody [EPR5784] (ab109527) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 40 kDa
    • ab109527, at 1/50, staining TIMM50 in paraffin-embedded kidney tissue by Immunohistochemistry.
    • ab109527, at 1/50, staining TIMM50 in paraffin-embedded liver tissue by Immunohistochemistry.

    Anti-TIMM50 antibody [EPR5784] (ab109527)参考文献

    ab109527 has not yet been referenced specifically in any publications.

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