概述

性能

应用

Our Abpromise guarantee covers the use of ab23938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/16000.
WB Use a concentration of 0.5 - 3 µg/ml. Detects a band of approximately 40 kDa.

靶标

  • 功能
    Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
  • 组织特异性
    Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
  • 序列相似性
    Belongs to the TIM50 family.
    Contains 1 FCP1 homology domain.
  • 结构域
    The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
  • 细胞定位
    Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homolog of yeast Tim50 antibody
    • Import inner membrane translocase subunit TIM 50 antibody
    • Import inner membrane translocase subunit TIM50 antibody
    • Import inner membrane translocase subunit TIM50 mitochondrial antibody
    • MGC102733 antibody
    • Mitochondrial import inner membrane translocase subunit TIM50 antibody
    • TIM 50 antibody
    • TIM 50L antibody
    • TIM50 antibody
    • Tim50 like protein antibody
    • TIM50_HUMAN antibody
    • TIM50L antibody
    • TIMM 50 antibody
    • TIMM50 antibody
    • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
    • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
    • Translocase of inner mitochondrial membrane 50 homolog antibody
    • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
    see all

图片

  • Anti-TIMM50 antibody (ab23938) at 0.5 µg/ml

    Performed under reducing conditions.

实验方案

文献

This product has been referenced in:
  • Abdel Malik R  et al. AMP-Activated Protein Kinase a2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1a and a Network of Proteins Affecting Metabolism and Apoptosis. Circ Res 120:99-109 (2017). WB ; Mouse . Read more (PubMed: 27777247) »
  • Byun J  et al. CR6-interacting factor 1 is a key regulator in Aß-induced mitochondrial disruption and pathogenesis of Alzheimer's disease. Cell Death Differ 22:959-73 (2015). WB ; Mouse . Read more (PubMed: 25361083) »

See all 6 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100/PBS
Blocking step
0.5% BSA, 0.2% fish skin gelatin/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 28 2010

Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount
25 µg
Specification
meiotic egg extract
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH/3T3 Fibroblasts)
Loading amount
20 µg
Specification
NIH/3T3 Fibroblasts
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HeLa post nuclear cell lysate)
Loading amount
30 µg
Specification
HeLa post nuclear cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

提交于 Aug 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册