Anti-TIM50抗体(ab23938)
Key features and details
- Goat polyclonal to TIM50
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TIM50抗体
参阅全部 TIM50 一抗 -
描述
山羊多克隆抗体to TIM50 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human TIM50 aa 293-306.
Sequence:(C)DVRTVLEHYALEDD
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阳性对照
- WB: human heart tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23938于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 40 kDa.
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 40 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. -
组织特异性
Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level). -
序列相似性
Belongs to the TIM50 family.
Contains 1 FCP1 homology domain. -
结构域
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo. -
细胞定位
Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs. - Information by UniProt
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数据库链接
- Entrez Gene: 505489 Cow
- Entrez Gene: 92609 Human
- Entrez Gene: 66525 Mouse
- Entrez Gene: 685725 Rat
- Omim: 607381 Human
- SwissProt: Q3SZB3 Cow
- SwissProt: Q3ZCQ8 Human
- SwissProt: Q9D880 Mouse
see all -
别名
- Homolog of yeast Tim50 antibody
- Import inner membrane translocase subunit TIM 50 antibody
- Import inner membrane translocase subunit TIM50 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab23938 被引用在 9 文献中.
- Farouk SM et al. Outer and inner mitochondrial membrane proteins TOMM40 and TIMM50 are intensively concentrated and localized at Purkinje and pyramidal neurons in the New Zealand white rabbit brain. Anat Rec (Hoboken) 305:209-221 (2022). PubMed: 34041863
- Kabashima A et al. Fibroblast growth factor receptor inhibition induces loss of matrix MCL1 and necrosis in cholangiocarcinoma. J Hepatol 68:1228-1238 (2018). WB . PubMed: 29408314
- Chen X et al. Artesunate Activates the Intrinsic Apoptosis of HCT116 Cells through the Suppression of Fatty Acid Synthesis and the NF-?B Pathway. Molecules 22:N/A (2017). PubMed: 28786914
- Abdel Malik R et al. AMP-Activated Protein Kinase a2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1a and a Network of Proteins Affecting Metabolism and Apoptosis. Circ Res 120:99-109 (2017). WB ; Mouse . PubMed: 27777247
- Byun J et al. CR6-interacting factor 1 is a key regulator in Aß-induced mitochondrial disruption and pathogenesis of Alzheimer's disease. Cell Death Differ 22:959-73 (2015). WB ; Mouse . PubMed: 25361083
- Baseler WA et al. Reversal of mitochondrial proteomic loss in Type 1 diabetic heart with overexpression of phospholipid hydroperoxide glutathione peroxidase. Am J Physiol Regul Integr Comp Physiol 304:R553-65 (2013). PubMed: 23408027
- Perciavalle RM et al. Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol 14:575-83 (2012). PubMed: 22544066
- Bigi A et al. Human sialidase NEU4 long and short are extrinsic proteins bound to outer mitochondrial membrane and the endoplasmic reticulum, respectively. Glycobiology 20:148-57 (2010). WB ; Human . PubMed: 19797320
- Goemans CG et al. Intra-mitochondrial degradation of Tim23 curtails the survival of cells rescued from apoptosis by caspase inhibitors. Cell Death Differ 15:545-54 (2008). WB . PubMed: 18174902