概述

  • 产品名称Anti-TIMM23抗体
  • 描述
    兔多克隆抗体to TIMM23
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human TIMM23 aa 130-158 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O14925

  • 阳性对照
    • WB: MCF7 and A431 cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab116329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 22 kDa.

靶标

  • 相关性TIMM23 belongs to the Tim17/Tim22/Tim23 family and is an essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
  • 细胞定位Mitochondrion inner membrane; Multi-pass membrane protein.
  • 数据库链接
  • 别名
    • MGC22767 antibody
    • MGC93478 antibody
    • Mitochondrial import inner membrane translocase subunit Tim23 antibody
    • Predicted protein of HQ1197 antibody
    • PRO1197 antibody
    • TIM23 antibody
    • TIMM 23 antibody
    • TIMM23 antibody
    • Translocase of inner mitochondrial membrane 23 (yeast) homolog antibody
    • Translocase of inner mitochondrial membrane 23 homolog (yeast) antibody
    • Translocase of inner mitochondrial membrane 23 homolog B antibody
    • Translocation of mitochondrial precursor proteins antibody
    see all

Anti-TIMM23 antibody 图像

  • All lanes : Anti-TIMM23 antibody (ab116329) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 22 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa


    Exposure time : 3 seconds

    Western blot analysis of NB4 human cell line (cell fractionation, 30µg/lane) labelling TIMM23 with ab116329 at 1/500.  ab97051 was used as the secondary antibody (1/10000).

    Treatment - DMSO Control (CTL), 0,5 mM Hydroxyurea (HU), 1 µM Doxorubicin (DR).

    See Abreview

Anti-TIMM23 antibody (ab116329)参考文献

This product has been referenced in:
  • Yano H  et al. Inhibition of mitochondrial protein import by mutant huntingtin. Nat Neurosci 17:822-31 (2014). ICC/IF ; Mouse . Read more (PubMed: 24836077) »
  • Zhang J  et al. UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells. EMBO J 30:4860-73 (2011). Read more (PubMed: 22085932) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (NB4 human cell line, cell fractionation)
Loading amount 30 µg
Specification NB4 human cell line, cell fractionation
Treatment DMSO Control (CTL), 0,5 mM Hydroxyurea (HU), 1 µM Doxorubicin (DR)
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

提交于 Feb 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"