Anti-TIMM13抗体(ab113781)

概述

  • 产品名称Anti-TIMM13抗体
    参阅全部 TIMM13 一抗
  • 描述
    兔多克隆抗体to TIMM13
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Chicken, Guinea pig, Cow, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N-terminal amino acids 5-54 (FGSDFGGTGG GKLDPGAIME QVKVQIAVAN AQELLQRMTD KCFRKCIGKP)of Mouse TIMM13 (NP_038923).

  • 阳性对照
    • Mouse Kidney Lysate

性能

应用

Our Abpromise guarantee covers the use of ab113781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins.
  • 组织特异性Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
  • 序列相似性Belongs to the small Tim family.
  • 结构域The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Mitochondrial import inner membrane translocase subunit TIM13 antibody
    • Mitochondrial import inner membrane translocase subunit Tim13B antibody
    • ppv1 antibody
    • TIM 13 antibody
    • TIM13 antibody
    • TIM13_HUMAN antibody
    • TIM13B antibody
    • TIMM 13 antibody
    • Timm13 antibody
    • TIMM13A antibody
    • TIMM13B antibody
    • Translocase of inner mitochondrial membrane 13 homolog antibody
    see all

Anti-TIMM13 antibody 图像

  • Anti-TIMM13 antibody (ab113781) at 1 µg/ml + Mouse Kidney Lysate at 10 µg

    Predicted band size : 11 kDa

Anti-TIMM13 antibody (ab113781)参考文献

ab113781 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart (Embryonic))
Permeabilization Yes - Tween-20
Specification Heart (Embryonic)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Aug 29 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 20 µg
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Aug 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"