Anti-Timeless抗体[EPR5275] (ab109512)


  • 产品名称Anti-Timeless抗体[EPR5275]
    参阅全部 Timeless 一抗
  • 描述
    兔单克隆抗体[EPR5275] to Timeless
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human Timeless.

  • 阳性对照
    • HeLa, Jurkat, JAR, and BxPC-3 cell lysates, Human colon and Human tonsil tissues
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/50000 - 1/200000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
    • 组织特异性Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis.
    • 序列相似性Belongs to the timeless family.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ12640 antibody
      • FLJ20714 antibody
      • hTIM antibody
      • Protein timeless homolog antibody
      • TIM antibody
      • TIM_HUMAN antibody
      • TIM1 antibody
      • Timeless antibody
      • timeless circadian clock 1 antibody
      • timeless circadian clock antibody
      • timeless homolog antibody
      • TIMELESS1 antibody
      • Tof1 homolog antibody
      see all

    Anti-Timeless antibody [EPR5275] 图像

    • Immunocytochemistry/Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma cell line) labeling Timeless with Purified ab109512 at 1/1000 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Timeless antibody [EPR5275] (ab109512) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 139 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109512 at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109512 at a dilution of 1/100.

    Anti-Timeless antibody [EPR5275] (ab109512)参考文献

    ab109512 has not yet been referenced specifically in any publications.

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