Anti-TIM 1抗体[mAbcam75295] (ab75295)
Key features and details
- Mouse monoclonal [mAbcam75295] to TIM 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TIM 1抗体[mAbcam75295]
参阅全部 TIM 1 一抗 -
描述
小鼠单克隆抗体[mAbcam75295] to TIM 1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human TIM 1 aa 300 to the C-terminus (C terminal).
(Peptide available asab97979) -
阳性对照
- This antibody gave a positive signal in the human testis and brain tissue lysates.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
mAbcam75295 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75295于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
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说明 |
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WB
Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa). |
靶标
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功能
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
组织特异性
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
序列相似性
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 26762 Human
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- Unigene: 129711 Human
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别名
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
see all
图片
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All lanes : Anti-TIM 1 antibody [mAbcam75295] (ab75295) at 10 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The band at approximately 50-kDa is comparable to the molecular weight seen with other antibodies to TIM 1. TIM 1 contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.
数据表及文件
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Datasheet download
文献 (0)
ab75295 尚未被引用在任何文献中。