概述

  • 产品名称Anti-TIM 1抗体
    参阅全部 TIM 1 一抗
  • 描述
    小鼠单克隆抗体to TIM 1
  • 经测试应用适用于: WB, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Recombinant fragment with tag: KVGGEAGPSV TLPCHYSGAV TSMCWNRGSC SLFTCQNGIV WTNGTHVTYR KDTRYKLLGD LSRRDVSLTI ENTAVSDSGV YCCRVEHRGW FNDMKITVSL, corresponding to amino acids 23-123 of Human TIM 1

  • 阳性对照
    • TIM 1 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab66062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent concentration. The detection limit for recombinant tagged TIM 1 is approximately 10ng/ml if used as a capture antibody.
Flow Cyt Use at an assay dependent concentration. See Abreview.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • 组织特异性Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • 序列相似性Belongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD365 antibody
    • HAVCR 1 antibody
    • HAVCR antibody
    • HAVcr-1 antibody
    • Havcr1 antibody
    • Hepatitis A virus cellular receptor 1 antibody
    • Kidney injury molecule 1 antibody
    • KIM 1 antibody
    • KIM-1 antibody
    • T cell immunoglobin domain and mucin domain protein 1 antibody
    • T cell immunoglobulin mucin family member 1 antibody
    • T cell immunoglobulin mucin receptor 1 antibody
    • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
    • T-cell membrane protein 1 antibody
    • TIM antibody
    • TIM-1 antibody
    • TIM1 antibody
    • TIMD 1 antibody
    • TIMD-1 antibody
    • TIMD1 antibody
    • TIMD1_HUMAN antibody
    see all

Anti-TIM 1 antibody 图像

  • All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml

    Lane 1 : TIM 1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 39 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

Anti-TIM 1 antibody (ab66062)参考文献

This product has been referenced in:
  • Liu J  et al. IL-10-producing B cells are induced early in HIV-1 infection and suppress HIV-1-specific T cell responses. PLoS One 9:e89236 (2014). Flow Cyt . Read more (PubMed: 24586620) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. We find that specific protocol is not necessary for this product. So I have attached the protocol that we generally recommend to our customers. We have used reduced denaturing conditions in WB without deglyco...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Peripheral blood mononuclear cells)
Specification Peripheral blood mononuclear cells
Preparation Cell harvesting/tissue preparation method: Ficoll gradient method
Sample buffer: PBS
Fixation none
Permeabilization No
Gating Strategy Lymphocytes and monocytes
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提交于 Aug 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"