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Our Abpromise guarantee covers the use of ab66062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).|
|ELISA||Use at an assay dependent concentration. The detection limit for recombinant tagged TIM 1 is approximately 10ng/ml if used as a capture antibody.|
|Flow Cyt||Use at an assay dependent concentration. See Abreview.
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.