概述

  • 产品名称
  • 描述
    兔多克隆抗体to TIGAR
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) of 19 amino acids from a region near the centre of TIGAR

  • 阳性对照
    • MCF7 cell lysate Human brain tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

靶标

  • 功能
    Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
  • 序列相似性
    Belongs to the phosphoglycerate mutase family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6-bisphosphatase TIGAR antibody
    • C12ORF5 antibody
    • chromosome 12 open reading frame 5 antibody
    • FR2BP antibody
    • Fructose-2,6-bisphosphatase TIGAR antibody
    • Fructose-2,6-bisphosphate 2-phosphatase antibody
    • Probable fructose 2,6 bisphosphatase TIGAR antibody
    • Probable fructose-2 antibody
    • tigar antibody
    • TIGAR_HUMAN antibody
    • TP53 induced glycolysis and apoptosis regulator antibody
    • TP53 induced glycolysis regulatory phosphatase antibody
    • TP53-induced glycolysis and apoptosis regulator antibody
    • Transactivated by NS3TP2 protein antibody
    see all

图片

  • Lane 1 : Anti-TIGAR antibody (ab62533) at 0.5 µg/ml
    Lane 2 : Anti-TIGAR antibody (ab62533) at 1 µg/ml
    Lane 3 : Anti-TIGAR antibody (ab62533) at 2 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • immunohistochemical analysis of TIGAR expression in human brain tissue using 2.5 µg/ml ab62533.
  • Immunofluorescence of TIGAR in Human Brain cells using ab62533 at 20 ug/ml.

文献

This product has been referenced in:
  • Maiti AK  et al. IL-4 Protects the Mitochondria Against TNFa and IFN? Induced Insult During Clearance of Infection with Citrobacter rodentium and Escherichia coli. Sci Rep 5:15434 (2015). IHC ; Mouse . Read more (PubMed: 26481427) »
  • Kim J  et al. TIGAR regulates glycolysis in ischemic kidney proximal tubules. Am J Physiol Renal Physiol 308:F298-308 (2015). Read more (PubMed: 25503731) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your inquiry.

Unfortunately, we cannot pinpoint exactly which of our antibodies was used for this publication.

With help of our records we can assume that it was ab62533

http://www.abcam.com/index.html?datash...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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