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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human TIGAR.
(Peptide available as ab37909.)
Our Abpromise guarantee covers the use of ab37910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
ICC/IF image of ab37910 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37910 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab37910 staining human adrenal tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C. A biotinylated goat anti-rabbit antibody diluted 1/400 was used as the secondary.