概述

  • 产品名称Anti-TIF1 gamma抗体
    参阅全部 TIF1 gamma 一抗
  • 描述
    兔多克隆抗体to TIF1 gamma
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 1080-1127 (IYSDRTFAPL PEFEQEEDDG EVTEDSDEDF IQPRRKRLKS DERPVHIK) of human TIF1 gamma (NP_056990).

  • 阳性对照
    • HeLa cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab86314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 123 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
  • 组织特异性Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.
  • 通路Protein modification; protein ubiquitination.
  • 疾病相关Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM33 is found in thyroid papillary carcinomas. Translocation t(1;10)(p13;q11) with RET. The translocation generates the TRIM33/RET (PTC7) oncogene.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • 细胞定位Nucleus. In discrete nuclear dots resembling nuclear bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 8030451N04Rik antibody
    • AI413936 antibody
    • cb1085 antibody
    • DKFZp586K1123 antibody
    • E3 ubiquitin-protein ligase TRIM33 antibody
    • EC 6.3.2.- antibody
    • Ectodermin antibody
    • Ectodermin homolog antibody
    • FLJ11429 antibody
    • FLJ32925 antibody
    • id:ibd2175 antibody
    • MGC136680 antibody
    • mKIAA1113 antibody
    • OTTHUMP00000013662 antibody
    • OTTHUMP00000013663 antibody
    • Protein Rfg7 antibody
    • PTC7 antibody
    • Ret fused gene 7 antibody
    • RET-fused gene 7 protein antibody
    • RFG7 antibody
    • Rfg7 protein antibody
    • TF1G antibody
    • TIF1-gamma antibody
    • TIF1G antibody
    • TIF1GAMMA antibody
    • TIFGAMMA antibody
    • Transcription intermediary factor 1-gamma antibody
    • Transcriptional intermediary factor 1 gamma antibody
    • TRI33_HUMAN antibody
    • trim33 antibody
    • Tripartite motif containing 33 antibody
    • Tripartite motif containing 33 protein antibody
    • tripartite motif-containing 33 antibody
    • Tripartite motif-containing protein 33 antibody
    • wu:fc17f10 antibody
    • zgc:136680 antibody
    see all

Anti-TIF1 gamma antibody 图像

  • Anti-TIF1 gamma antibody (ab86314) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 123 kDa
    Observed band size : ~123 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 145 kDa. We are unsure as to the identity of these extra bands.

Anti-TIF1 gamma antibody (ab86314)参考文献

ab86314 has not yet been referenced specifically in any publications.

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