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Our Abpromise guarantee covers the use of ab24859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/25. PubMed: 19253934|
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 126 kDa.|
|ELISA||Use a concentration of 1 - 2 µg/ml.|
|Flow Cyt||Use 1-2µg for 106 cells.
We tested in-house with methanol-fixed cells, but this may not be necessary since the antibody recognies an extracellular epitope.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration. PubMed: 20622121|