Anti-TIE1抗体(ab111547)

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概述

  • 产品名称
    Anti-TIE1抗体
    参阅全部 TIE1 一抗
  • 描述
    兔多克隆抗体to TIE1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 677 and 1021 (P35590) of TIE1

  • 阳性对照
    • A549 lysate, HeLa lysate

性能

应用

Our Abpromise guarantee covers the use of ab111547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 125 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0

靶标

  • 功能
    Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
  • 组织特异性
    Specifically expressed in developing vascular endothelial cells.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • JKT 14 antibody
    • JTK14 antibody
    • TIE antibody
    • Tie1 antibody
    • TIE1_HUMAN antibody
    • Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody
    • Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody
    • Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody
    • Tyrosine protein kinase receptor Tie 1 antibody
    • Tyrosine-protein kinase receptor Tie-1 antibody
    see all

图片

  • Western blot - Anti-TIE1 antibody (ab111547)
    Western blot - Anti-TIE1 antibody (ab111547)
    Anti-TIE1 antibody (ab111547) at 1/1000 dilution + A549 lysate at 30 µg/ml

    Predicted band size: 125 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (ab111547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (ab111547)
    Immunohistochemical analysis of paraffin embedded Colon carcinoma, using ab111547 at 1:500 dilution.

文献

ab111547 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab111547？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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3 reviews or Q&A

10
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Dear All,

I would like to know whether Tie1 antibody (ab111547) specifically recognizes only Tie1 or there is also cross-reactivity with Tie2?

Thank you for contacting us.

The immunogen for this product shares about 70% homology and so is not likely to react. Typically at about 85% there is likely reactivity.

I hope this information is helpful to you. Please do not hesitate ...

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Dear Technical Team,

Thank you for your mail.

Kindly note that the customer used this antibody in Human Tumor cells.

Looking forward towards your valued reply on it.

With Best Regards,

Thank you for kindly confirming these details.

As previously requested by the customer, we would be pleased to arrangea free of charge replacement. In order to do this, I am forwarding these details on to our distributors team who will be ha...

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Dear Technical Team,

Hope this e-mail finds you well!

Please find below a complaint from one of our customerto whom we suppliedantibody having cat no ab111547.

The technical formis alsoattachedwith this mail.

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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