Anti-TIE1抗体(ab111547)
Key features and details
- Rabbit polyclonal to TIE1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TIE1抗体
参阅全部 TIE1 一抗 -
描述
兔多克隆抗体to TIE1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 677 and 1021 (P35590) of TIE1
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阳性对照
- A549 lysate, HeLa lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111547于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 125 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 125 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0 |
靶标
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功能
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. -
组织特异性
Specifically expressed in developing vascular endothelial cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 280941 Cow
- Entrez Gene: 7075 Human
- Entrez Gene: 21846 Mouse
- Entrez Gene: 89806 Rat
- Omim: 600222 Human
- SwissProt: Q06805 Cow
- SwissProt: P35590 Human
- SwissProt: Q06806 Mouse
see all -
别名
- JKT 14 antibody
- JTK14 antibody
- TIE antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab111547 被引用在 4 文献中.
- Helms EJ et al. Mesenchymal Lineage Heterogeneity Underlies Nonredundant Functions of Pancreatic Cancer-Associated Fibroblasts. Cancer Discov 12:484-501 (2022). PubMed: 34548310
- Zhang J et al. Dual inhibition of PFKFB3 and VEGF normalizes tumor vasculature, reduces lactate production, and improves chemotherapy in glioblastoma: insights from protein expression profiling and MRI. Theranostics 10:7245-7259 (2020). PubMed: 32641990
- Zhang X et al. Potential of Tyrosine Kinase Receptor TIE-1 as Novel Therapeutic Target in High-PI3K-Expressing Ovarian Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32604863
- Huang R et al. Metastatic pathway and the microvascular and physicochemical microenvironments of human melanoma xenografts. J Transl Med 15:203 (2017). IHC ; Mouse . PubMed: 29017512