Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Western blot - Anti-TIE1 antibody [8C9] (ab27851)
All lanes : Anti-TIE1 antibody [8C9] (ab27851)
Lane 1 : Recombinant human soluble TIE1 Lane 2 : Recombinant human soluble TIE2 Lane 3 : Recombinant mouse soluble TIE1 Lane 4 : Recombinant mouse soluble TIE2