概述

  • 产品名称Anti-TID1抗体[RS13]
    参阅全部 TID1 一抗
  • 描述
    小鼠单克隆抗体[RS13] to TID1
  • 特异性Reacts with both short and long variants of TID1.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant human TID1 protein.

  • 阳性对照
    • HeLa and Jurkat cells

性能

应用

Our Abpromise guarantee covers the use of ab81700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2 µg/mg of lysate. Co precipitates mitochondrial Hsp70.
    WB: Use at a concentration of 1 - 2 µg/ml for 2 hours at room temperature. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性TID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.
    • 细胞定位Mitochondrial
    • 数据库链接
    • 别名
      • DnaJ (Hsp40) homolog subfamily A member 3 antibody
      • DnaJ homolog subfamily A member 3 mitochondrial precursor antibody
      • DnaJ protein Tid 1 antibody
      • DnaJ protein Tid1 antibody
      • FLJ45758 antibody
      • HCA57 antibody
      • Hepatocellular carcinoma associated antigen 57 antibody
      • Highly similar to HYPOTHETICAL 105.9 KD PROTEIN F22B7.5 IN CHROMOSOME antibody
      • hTid 1 antibody
      • hTid1 antibody
      • III [Caenorhabditis elegans] antibody
      • TID1 antibody
      • Tumorous imaginal discs (Drosophila) homolog antibody
      • Tumorous imaginal discs protein Tid56 homolog antibody
      see all

    Anti-TID1 antibody [RS13] 图像

    Anti-TID1 antibody [RS13] (ab81700)参考文献

    This product has been referenced in:
    • Proft J  et al. Identification of Bilateral Changes in TID1 Expression in the 6-OHDA Rat Model of Parkinson's Disease. PLoS One 6:e26045 (2011). WB, IHC-Fr ; Rat . Read more (PubMed: 22016808) »
    • Syken J  et al. TID1, a human homolog of the Drosophila tumor suppressor l(2)tid, encodes two mitochondrial modulators of apoptosis with opposing functions. Proc Natl Acad Sci U S A 96:8499-504 (1999). Human . Read more (PubMed: 10411904) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (293T kidney cells)
    Total protein in input 150 µg
    Specification 293T kidney cells
    Treatment tranfected with Tid1-flag
    Immuno-precipitation step Protein G
    Username

    Mr. Christian Marx

    Verified customer

    提交于 Dec 14 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (HCT116 colon cancer cell line)
    Loading amount 60 µg
    Specification HCT116 colon cancer cell line
    Treatment different treatments
    Gel Running Conditions Reduced Denaturing (13%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Christian Marx

    Verified customer

    提交于 Oct 09 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"