概述

  • 产品名称Anti-TID1抗体
    参阅全部 TID1 一抗
  • 描述
    兔多克隆抗体to TID1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH corresponding to C-terminal residues of Mouse TID1 protein.

  • 阳性对照
    • Human skeletal nuscle.

性能

应用

Our Abpromise guarantee covers the use of ab115697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性TID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.
  • 细胞定位Mitochondrial
  • 数据库链接
  • 别名
    • DnaJ (Hsp40) homolog subfamily A member 3 antibody
    • DnaJ homolog subfamily A member 3 mitochondrial precursor antibody
    • DnaJ protein Tid 1 antibody
    • DnaJ protein Tid1 antibody
    • FLJ45758 antibody
    • HCA57 antibody
    • Hepatocellular carcinoma associated antigen 57 antibody
    • Highly similar to HYPOTHETICAL 105.9 KD PROTEIN F22B7.5 IN CHROMOSOME antibody
    • hTid 1 antibody
    • hTid1 antibody
    • III [Caenorhabditis elegans] antibody
    • TID1 antibody
    • Tumorous imaginal discs (Drosophila) homolog antibody
    • Tumorous imaginal discs protein Tid56 homolog antibody
    see all

Anti-TID1 antibody 图像

  • Immunohistochemistry Image of Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with TID1 antibody (ab115697) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-TID1 antibody (ab115697)参考文献

ab115697 has not yet been referenced specifically in any publications.

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