The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 24 kDa.
功能Functions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1.
组织特异性Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas.
序列相似性Contains 1 TIR domain.
结构域The TIR domain mediates the interaction with TRAF6.
翻译后修饰Phosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation. Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.
细胞定位Cytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane.
ab17221 at 10µg/ml staining TICAM2 in Raji cells by ICC/IF
Anti-TICAM2 antibody (ab17221)参考文献
This product has been referenced in:
Endo Y et al. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1ß and TRIF/IRF3. PLoS One9:e98517 (2014).
Read more (PubMed: 24870145) »