概述

  • 产品名称
    Anti-TIA1抗体[TIA-1]
    参阅全部 TIA1 一抗
  • 描述
    小鼠单克隆抗体[TIA-1] to TIA1
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 阳性对照
    • IHC-P: Human spleen tissue.
  • 常规说明

    TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Primary antibody说明
    TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.
  • 克隆
    单克隆
  • 克隆编号
    TIA-1
  • 骨髓瘤
    unknown
  • 同种型
    IgG1
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Incubate for 30-60 minutes at RT. Use heat-mediated antigen retrieval, an optional pepsin treatment can also improve the signal.

靶标

  • 功能
    Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
  • 序列相似性
    Contains 3 RRM (RNA recognition motif) domains.
  • 细胞定位
    Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytotoxic granule associated RNA binding protein 1 antibody
    • Cytotoxic granule associated RNA binding protein antibody
    • mTIA-1 antibody
    • Nucleolysin TIA 1 isoform p40 antibody
    • Nucleolysin TIA-1 isoform p40 antibody
    • Nucleolysin TIA1 isoform p40 antibody
    • p40 TIA 1 antibody
    • p40-TIA-1 (containing p15-TIA-1) antibody
    • p40-TIA-1 antibody
    • RNA binding protein TIA 1 antibody
    • RNA binding protein TIA1 antibody
    • RNA-binding protein TIA-1 antibody
    • T-cell-restricted intracellular antigen-1 antibody
    • TIA 1 antibody
    • TIA 1 cytotoxic granule associated RNA binding protein antibody
    • Tia antibody
    • TIA-1 antibody
    • TIA1 antibody
    • TIA1 cytotoxic granule associated RNA binding protein antibody
    • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
    • TIA1 protein antibody
    • TIA1_HUMAN antibody
    • TIAL1 antibody
    • TIAR antibody
    • WDM antibody
    see all

图片

  • Human normal spleen. Staining is observed in the cytoplasm. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system at room temperature: sections were rehydrated and antigen retrieved with buffers citrate EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (casein 0.25% in PBS) then incubated with primary antibody for 20 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

文献

This product has been referenced in:
  • Ghosh S & Geahlen RL Stress Granules Modulate SYK to Cause Microglial Cell Dysfunction in Alzheimer's Disease. EBioMedicine 2:1785-98 (2015). WB, ICC/IF ; Mouse . Read more (PubMed: 26870803) »
  • van Gool IC  et al. POLE Proofreading Mutations Elicit an Antitumor Immune Response in Endometrial Cancer. Clin Cancer Res 21:3347-55 (2015). IHC-P ; Human . Read more (PubMed: 25878334) »

See all 13 Publications for this product

客户评价及客户问答

Thank you for contacting us.We have not teted the specificity, the only information we have available is that the TIA-1 antibody reaacts with a 15 kDa cytoplasmic granule-associated protein. Please let me know if you have any further questions.

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (neuroblastoma)
Specification
neuroblastoma
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X 100
Blocking step
goat serum,0.5%BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 02 2012

Thank you very much for your email.

One vial of ab2712 has been added to your order, and you can pay for this order with the PO that you provided or with a credit card if you prefer. If you'd like to add a credit card to your order, please ca...

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DISCOUNT CODE: ***
Expiration date: January 5th, 2012

I am very pleased to hear you would like to accept our offer and test ab2712 in ICC. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer...

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Application
Western blot
Sample
Spermophilus tridecemlineatus Tissue lysate - whole (Liver, Heart)
Loading amount
30 µg
Specification
Liver, Heart
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
PVA as blocking agent for 1 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 06 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Renal proximal tubular epithelial cells)
Specification
Renal proximal tubular epithelial cells
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Dr. Robert Jenkins

Verified customer

提交于 Apr 13 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS 7 cells)
Specification
COS 7 cells
Fixative
Methanol
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3
Username

Mrs. Gabriela Elena Oprea

Verified customer

提交于 Aug 16 2006

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary cultured hippocampal neurons, COS)
Specification
primary cultured hippocampal neurons, COS
Fixative
Paraformaldehyde
Blocking step
Other as blocking agent for 30 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Jun 05 2006

Thank you for your enquiry. The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section. We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for ...

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Thank you for taking the time to submit a review to our system. I am taking your comments seriously and would appreciate your assistance to enable me to look into the source of the problem in more detail. Could you fill out the questionnaire at the f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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