概述

  • 产品名称
  • 描述
    山羊多克隆抗体to TIA1
  • 特异性
    This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    C-PKSTYESNTKQ

    , corresponding to internal sequence amino acids 189-199 of Human TIA1

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab61700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    Peptide ELISA: antibody detection limit dilution 1:128,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

    An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
    • 序列相似性
      Contains 3 RRM (RNA recognition motif) domains.
    • 细胞定位
      Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cytotoxic granule associated RNA binding protein 1 antibody
      • Cytotoxic granule associated RNA binding protein antibody
      • mTIA-1 antibody
      • Nucleolysin TIA 1 isoform p40 antibody
      • Nucleolysin TIA-1 isoform p40 antibody
      • Nucleolysin TIA1 isoform p40 antibody
      • p40 TIA 1 antibody
      • p40-TIA-1 (containing p15-TIA-1) antibody
      • p40-TIA-1 antibody
      • RNA binding protein TIA 1 antibody
      • RNA binding protein TIA1 antibody
      • RNA-binding protein TIA-1 antibody
      • T-cell-restricted intracellular antigen-1 antibody
      • TIA 1 antibody
      • TIA 1 cytotoxic granule associated RNA binding protein antibody
      • Tia antibody
      • TIA-1 antibody
      • TIA1 antibody
      • TIA1 cytotoxic granule associated RNA binding protein antibody
      • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
      • TIA1 protein antibody
      • TIA1_HUMAN antibody
      • TIAL1 antibody
      • TIAR antibody
      • WDM antibody
      see all

    Anti-TIA1 antibody 图像

    • Anti-TIA1 antibody (ab61700) at 0.1 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).

      Predicted band size : 43 kDa
      Observed band size : 43 kDa
      Additional bands at : 26 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    Anti-TIA1 antibody (ab61700)参考文献

    ab61700 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (HEK293T)
    Permeabilization
    Yes - Triton X100
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Sukhleen Kour

    Verified customer

    提交于 Feb 21 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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