Anti-Thyroxine Binding Globulin抗体(ab48646)

概述

  • 产品名称Anti-Thyroxine Binding Globulin抗体
    参阅全部 Thyroxine Binding Globulin 一抗
  • 描述
    兔多克隆抗体to Thyroxine Binding Globulin
  • 经测试应用适用于: RIA, IP, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Dog, Human
  • 免疫原

    Human TBG purified from human plasma

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab48646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
RIA
IP
WB
ELISA
ICC/IF
  • 应用说明EIA: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 46 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • Information by UniProt
    • 数据库链接
    • 别名
      • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 antibody
      • Serpin A7 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 antibody
      • Serpin peptidase inhibitor, clade A, member 7 antibody
      • Serpina7 antibody
      • T4 binding globulin antibody
      • TBG antibody
      • TBG, serum antibody
      • THBG_HUMAN antibody
      • Thyroxin binding globulin antibody
      • Thyroxine binding globulin antibody
      • Thyroxine-binding globulin antibody
      • Thyroxine-binding globulin od serum antibody
      see all

    Anti-Thyroxine Binding Globulin antibody 图像

    • ICC/IF image of ab48646 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48646, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-Thyroxine Binding Globulin antibody (ab48646) at 1 µg/ml + Natural Human Thyroxine Binding Globulin protein (ab96516) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 90 seconds

    Anti-Thyroxine Binding Globulin antibody (ab48646)参考文献

    ab48646 has not yet been referenced specifically in any publications.

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