重组Anti-Thyroid Peroxidase/TPO抗体[EPR5379] (ab133322)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5379] to Thyroid Peroxidase/TPO
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Thyroid Peroxidase/TPO抗体[EPR5379]
参阅全部 Thyroid Peroxidase/TPO 一抗 -
描述
兔单克隆抗体[EPR5379] to Thyroid Peroxidase/TPO -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human thyroid lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5379 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/10000 - 1/20000. Predicted molecular weight: 103 kDa.
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IP |
1/40.
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说明 |
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IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/10000 - 1/20000. Predicted molecular weight: 103 kDa. |
IP
1/40. |
靶标
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功能
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). -
通路
Hormone biosynthesis; thyroid hormone biosynthesis. -
疾病相关
Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. -
序列相似性
Belongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
翻译后修饰
Glycosylated.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part. -
细胞定位
Membrane and Cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 7173 Human
- Omim: 606765 Human
- SwissProt: P07202 Human
- Unigene: 467554 Human
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别名
- MSA antibody
- PERT_HUMAN antibody
- TDH2A antibody
see all
图片
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Immunohistochemical staining of paraffin embedded human thyroid with purified ab133322 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/20000 dilution (purified) + Human thyroid tissue lysate at 10 µg
Secondary
HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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ab133322 (purified) at 1/40 immunoprecipitating thyroid peroxidase/TPO in human thyroid tissue lysate (Lane 1). For western blotting, a HRP-conjugated goat anti-rabbit IgG, was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/1000 dilution (unpurified) + Human thyroid lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 103 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab133322 被引用在 4 文献中.
- Liu Y et al. Pyruvate carboxylase promotes malignant transformation of papillary thyroid carcinoma and reduces iodine uptake. Cell Death Discov 8:423 (2022). PubMed: 36266265
- Cui X et al. Circulating Exosomes Activate Dendritic Cells and Induce Unbalanced CD4+ T Cell Differentiation in Hashimoto Thyroiditis. J Clin Endocrinol Metab 104:4607-4618 (2019). PubMed: 31199456
- Guria S et al. Functional analysis of thyroid peroxidase gene mutations detected in patients with thyroid dyshormonogenesis. Int J Endocrinol 2014:390121 (2014). PubMed: 24829575
- Ishido Y et al. Thyroglobulin suppresses thyroid-specific gene expression in cultures of normal but not neoplastic human thyroid follicular cells. J Clin Endocrinol Metab 99:E694-702 (2014). PubMed: 24433000