Anti-Thyroid Peroxidase抗体(ab76935)

概述

  • 产品名称Anti-Thyroid Peroxidase抗体
    参阅全部 Thyroid Peroxidase 一抗
  • 描述
    小鼠单克隆抗体to Thyroid Peroxidase
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 672-780 of Human Thyroid Peroxidase with a 26kDa tag.

  • 阳性对照
    • Transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab76935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Thyroid Peroxidase is approximately 1ng/ml when used as a capture antibody.

靶标

  • 功能Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • 通路Hormone biosynthesis; thyroid hormone biosynthesis.
  • 疾病相关Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • 序列相似性Belongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • 翻译后修饰Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • 细胞定位Membrane and Cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MSA antibody
    • PERT_HUMAN antibody
    • TDH2A antibody
    • Thyroid microsomal antigen antibody
    • Thyroid peroxidase antibody
    • Thyroperoxidase antibody
    • TPO antibody
    • TPX antibody
    see all

Anti-Thyroid Peroxidase antibody 图像

  • Anti-Thyroid Peroxidase antibody (ab76935) at 5 µg/ml + Immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Thyroid Peroxidase antibody (ab76935) at 5 µg/ml

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : 103 kDa
  • Capture ELISA using ab76935 at 10ug/ml.

Anti-Thyroid Peroxidase antibody (ab76935)参考文献

ab76935 has not yet been referenced specifically in any publications.

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