Anti-Thyroid Peroxidase抗体(ab123805)

概述

  • 产品名称Anti-Thyroid Peroxidase抗体
    参阅全部 Thyroid Peroxidase 一抗
  • 描述
    山羊多克隆抗体to Thyroid Peroxidase
  • 特异性ab123805 is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1).
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    ELRCGKHQAVGTSPQ

    , corresponding to C terminal amino acids 893-907 of Human Thyroid Peroxidase (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1).

  • 阳性对照
    • Human thyroid gland lysate.

性能

应用

Our Abpromise guarantee covers the use of ab123805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 103 kDa.

靶标

  • 功能Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • 通路Hormone biosynthesis; thyroid hormone biosynthesis.
  • 疾病相关Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • 序列相似性Belongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • 翻译后修饰Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • 细胞定位Membrane and Cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MSA antibody
    • PERT_HUMAN antibody
    • TDH2A antibody
    • Thyroid microsomal antigen antibody
    • Thyroid peroxidase antibody
    • Thyroperoxidase antibody
    • TPO antibody
    • TPX antibody
    see all

Anti-Thyroid Peroxidase antibody 图像

  • ab123805 (5µg/ml) staining of paraffin embedded Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured staining in the cytoplasm of epithelial cells.

  • Anti-Thyroid Peroxidase antibody (ab123805) at 0.1 µg/ml + Thyroid Gland lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 103 kDa

Anti-Thyroid Peroxidase antibody (ab123805)参考文献

ab123805 has not yet been referenced specifically in any publications.

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