Anti-Thyroid Hormone Receptor beta抗体[J52] (ab2744)

概述

  • 产品名称Anti-Thyroid Hormone Receptor beta抗体[J52]
    参阅全部 Thyroid Hormone Receptor beta 一抗
  • 描述
    小鼠单克隆抗体[J52] to Thyroid Hormone Receptor beta
  • 特异性Detects thyroid hormone receptor (TR) beta in human, rat and transformed bacterial cells. This antibody is specific for the TR beta-1 isoform. This antibody detects a 52 and a 55 kDa protein representing recombinant TR expressed in E. coli. Immunocytochemical staining of TR beta-1 in intact GH3 cells with this antibody results in nuclear staining. When levels of expression of the transfected receptor are high, cytoplasmic staining is seen along with nuclear staining. Immunoprecipitation and gel super shift experiments show that this antibody reacts with the unliganded, liganded, and DNA binding forms of the receptor.
  • 经测试应用适用于: ICC, IP, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Purified human THR beta overexpressed in E. coli

  • 表位This antibody recognizes an epitope in the A/B domain of human THR beta, amino acid residues 1-101.
  • 阳性对照
    • WB: recombinant protein expressed in E. coli ICC: GH3 rat cells
  • 常规说明


    A profound increase in expression of beta receptors is observed in the brain shortly after birth. The beta receptor activates expression of several genes known to be important in brain development (e.g. myelin basic protein), implicating it in the well characterised effects of thyroid hormones on development of the fetal and neonatal brain.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS with 0.05% sodium azide
  • Concentration information loading...
  • Primary antibody说明A profound increase in expression of beta receptors is observed in the brain shortly after birth. The beta receptor activates expression of several genes known to be important in brain development (e.g. myelin basic protein), implicating it in the well characterised effects of thyroid hormones on development of the fetal and neonatal brain.
  • 克隆单克隆
  • 克隆编号J52
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/500.

Fix cells with 3.7% formaldehyde.

IP Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 52-55 kDa (predicted molecular weight: 52 kDa).
EMSA Use at an assay dependent concentration.

靶标

  • 功能High affinity receptor for triiodothyronine.
  • 疾病相关Defects in THRB are the cause of generalized thyroid hormone resistance (GTHR) [MIM:188570, 274300]. GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).
    Defects in THRB are the cause of selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]; also known as familial hyperthyroidism due to inappropriate thyrotropin secretion. PRTH is a variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 antibody
    • C ERBA 2 antibody
    • C ERBA BETA antibody
    • c-erbA-2 antibody
    • c-erbA-beta antibody
    • ERBA 2 antibody
    • ERBA BETA antibody
    • ERBA2 antibody
    • Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian antibody
    • generalized resistance to thyroid hormone antibody
    • GRTH antibody
    • MGC126109 antibody
    • MGC126110 antibody
    • NR1A2 antibody
    • Nuclear receptor subfamily 1 group A member 2 antibody
    • Oncogene ERBA2 antibody
    • PRTH antibody
    • THB_HUMAN antibody
    • THR1 antibody
    • THRB 1 antibody
    • THRB 2 antibody
    • thrB antibody
    • THRB1 antibody
    • THRB2 antibody
    • Thyroid hormone nuclear receptor beta variant 1 antibody
    • Thyroid hormone receptor beta 1 antibody
    • Thyroid hormone receptor beta 2 antibody
    • Thyroid hormone receptor beta antibody
    • Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian) antibody
    see all

Anti-Thyroid Hormone Receptor beta antibody [J52] 图像



  • Predicted band size : 52 kDa


    Western blot of human thyroid hormone receptor beta using ab2744.

Anti-Thyroid Hormone Receptor beta antibody [J52] (ab2744)参考文献

This product has been referenced in:
  • Liu Y  et al. p62, A TFIIH subunit, directly interacts with thyroid hormone receptor and enhances T3-mediated transcription. Mol Endocrinol 19:879-84 (2005). Read more (PubMed: 15625236) »
  • Meier CA  et al. Variable transcriptional activity and ligand binding of mutant beta 1 3,5,3'-triiodothyronine receptors from four families with generalized resistance to thyroid hormone. Mol Endocrinol 6:248-58 (1992). Read more (PubMed: 1569968) »

See all 3 Publications for this product

Product Wall

I have been informed by the laboratory that ab2744 has only been tested in our lab in WB on recombinant protein expressed in E. coli. The following reference has used this antibody in WB too: Liu Y et al. p62, A TFIIH subunit, directly interacts wi...

Read More

I'm sorry to hear you are having problems with ab2744. I am in touch with the laboratory that tested the antibody to find out the recommended positive control as I am concerned your samples may express low levels of Thyroid Hormone Receptor beta 1....

Read More

Thank you for your enquiry and your interest in our products. The TRB1 antibody will only detect the transfected receptor. Endogenous expression will most likely yield poor results.

Thank you for your enquiry. Unfortunately, we don't have the corresponding peptides for these antibodies. If you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's Antibody Gateway". The World's ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"