Anti-Thyroid Hormone Receptor alpha 1+2抗体(ab42565)

概述

  • 产品名称
    Anti-Thyroid Hormone Receptor alpha 1+2抗体
    参阅全部 Thyroid Hormone Receptor alpha 1+2 一抗
  • 描述
    兔多克隆抗体to Thyroid Hormone Receptor alpha 1+2
  • 经测试应用
    适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig, Zebrafish
  • 免疫原

    A peptide corresponding to a region of Human THRA

  • 阳性对照
    • Jurkat cell lysate and human skin tissue

性能

应用

Our Abpromise guarantee covers the use of ab42565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 58, 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

ICC/IF 1/100.

靶标

图片

  • Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58, 47 kDa
    Observed band size : 47,58 kDa
  • ab42565 at 4ug/ml concentration, staining human THRA in squamous epithelial cells of human skin (indicated by arrows) by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

文献

ab42565 has not yet been referenced specifically in any publications.

客户评价及客户问答



DISCOUNT CODE: ***
Expiration date: November 16th, 2012

I am very pleased to hear you would like to accept our offer and test ab42565 in ChIP. This code will give you: 1 freeprimary antibody before the expiration date. To redee...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human TRa expressed in CHO-K1)
Specification
Human TRa expressed in CHO-K1
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

提交于 Aug 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册