Anti-Thymosin beta 4抗体(ab14335)

概述

  • 产品名称Anti-Thymosin beta 4抗体
    参阅全部 Thymosin beta 4 一抗
  • 描述
    兔多克隆抗体to Thymosin beta 4
  • 特异性Human Thymosin B4 (aa 1-11)
  • 经测试应用适用于: ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Chicken, Human
  • 免疫原

    Synthetic peptide:

    SDKPDMAEIEK

    conjugated to KLH, corresponding to amino acids 1-11 of Human Thymosin beta 4.

  • 阳性对照
    • ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab14335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
ICC/IF 1/200.
IHC-P 1/1000.

Tested in lung and heart tissues.

靶标

  • 功能Plays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
    Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase.
  • 组织特异性Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.
  • 序列相似性Belongs to the thymosin beta family.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Fx antibody
    • Hematopoietic system regulatory peptide antibody
    • Prothymosin beta 4 antibody
    • PTMB 4 antibody
    • PTMB4 antibody
    • Seraspenide antibody
    • T beta 4 antibody
    • T beta-4 antibody
    • TB4X antibody
    • THYB 4 antibody
    • Thyb4 antibody
    • Thymosin beta 4 antibody
    • Thymosin beta 4 X chromosome antibody
    • Thymosin beta 4 X linked antibody
    • TMSB 4 antibody
    • TMSB4 antibody
    • Tmsb4x antibody
    • TYB4_HUMAN antibody
    see all

Anti-Thymosin beta 4 antibody 图像

  • ICC/IF image of ab14335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14335, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14335 at 1/1000 dilution.

Anti-Thymosin beta 4 antibody (ab14335)参考文献

This product has been referenced in:
  • Wong SW  et al. H2O 2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization. Ann Biomed Eng 43:1178-88 (2015). Read more (PubMed: 25371376) »
  • Fu X  et al. Thymosin ß4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition. Mol Med Rep 12:127-32 (2015). IHC ; Human . Read more (PubMed: 25695679) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample Xenopus laevis Cell (Brain)
Specification Brain
Permeabilization Yes - 0.01% Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 27 2014

Application ELISA
Sample Chicken Serum (heart culture)
Specification heart culture
Type Indirect
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Yanyang Li

Verified customer

提交于 Jan 13 2014

DISCOUNT CODE: xxxxxxx
Expiration date: 10th May 2013
Value: £235

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for ab101672:
Antigen retrieval: Sodium citrate buffer (10 mM sodium citrate, pH 6.0), boil for 15 minutes.
Positive control: hepatocarcinoma .


for ab14335:
No antigen retrieval is necessary, the protocol is as follows: Read More

Thank you for contacting us.

For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

For ab1215...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"