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Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Thymine DNA glycosylase .
Our Abpromise guarantee covers the use of ab106301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).|
|IP||Use a concentration of 5 µg/ml.|
Thymine DNA glycosylase was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Thymine DNA glycosylase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab106301.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 50kDa; Thymine DNA glycosylase
This image is courtesy of an anonymous Abreview
ab106301 has not yet been referenced specifically in any publications.