Anti-Thymine DNA glycosylase抗体(ab106301)

概述

  • 产品名称Anti-Thymine DNA glycosylase抗体
    参阅全部 Thymine DNA glycosylase 一抗
  • 描述
    兔多克隆抗体to Thymine DNA glycosylase
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Thymine DNA glycosylase .

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; A431; Hues7.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).
IP Use a concentration of 5 µg/ml.

靶标

  • 功能In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
  • 序列相似性Belongs to the TDG/mug DNA glycosylase family.
  • 翻译后修饰Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C JUN leucine zipper interactive protein antibody
    • C JUN leucine zipper interactive protein antibody
    • C-JUN leucine zipper interactive protein JZA-3 antibody
    • E130317C12Rik antibody
    • EC 3.2.2.29 antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch-specific thymine DNA glycosylase antibody
    • JZA 3 antibody
    • Jza1 antibody
    • T:G mismatch thymine glycosylase antibody
    • Tdg antibody
    • TDG_HUMAN antibody
    • Thymine DNA glycosylase antibody
    • Thymine-DNA glycosylase antibody
    see all

Anti-Thymine DNA glycosylase antibody 图像

  • Thymine DNA glycosylase was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Thymine DNA glycosylase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab106301.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 50kDa; Thymine DNA glycosylase

  • All lanes : Anti-Thymine DNA glycosylase antibody (ab106301) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • All lanes : Anti-Thymine DNA glycosylase antibody (ab106301) at 1 µg/ml

    Lane 1 : Mouse ES whole cell lysate
    Lane 2 : Mouse ES whole cell lysate

    Lysates/proteins at 100 µg per lane.

    Secondary
    HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Thymine DNA glycosylase antibody (ab106301)参考文献

ab106301 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (10)
Sample Human Cell lysate - whole cell (Prostate, Kidney)
Specification Prostate, Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Nov 14 2013

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Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Embryonic stem cell)
Loading amount 100 µg
Specification Embryonic stem cell
Gel Running Conditions Reduced Denaturing (8% page gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"