重组
RabMAb

Anti-Thymidylate Synthase抗体[EPR4545] (ab108995)

概述

  • 产品名称
    Anti-Thymidylate Synthase抗体[EPR4545]
    参阅全部 Thymidylate Synthase 一抗
  • 描述
    兔单克隆抗体[EPR4545] to Thymidylate Synthase
  • 经测试应用
    适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Thymidylate Synthase aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P04818

  • 阳性对照
    • HeLa, Jurkat, HL60 and MCF7 cell lysates; Human ovary carcinoma tissue.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

图片

  • All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 36 kDa
  • ab108995 at 1/50 dilution, staining Thymidylate Synthase in Human ovary carcinoma Immunohistochemistry, Paraffin-embedded tissue.

文献

This product has been referenced in:
  • Monica V  et al. Dasatinib modulates sensitivity to pemetrexed in malignant pleural mesothelioma cell lines. Oncotarget 7:76577-76589 (2016). WB ; Human . Read more (PubMed: 27391433) »
  • Lund K  et al. Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment. PLoS One 10:e0123684 (2015). WB . Read more (PubMed: 25860483) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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