Anti-Thymidylate Synthase抗体(ab7398)

Submit an Abreview Q&A (6)Specific References (6)

概述

  • 产品名称
    Anti-Thymidylate Synthase抗体
    参阅全部 Thymidylate Synthase 一抗
  • 描述
    羊多克隆抗体to Thymidylate Synthase
  • 宿主
    Sheep
  • 经测试应用
    适用于: ELISA, IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Escherichia coli, African green monkey
    不与反应: Saccharomyces cerevisiae
  • 免疫原

    Recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

  • 阳性对照
    • HeLa cells treated with the TS inhibitor 5-FUdR
  • 常规说明
    The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.

性能

应用

Our Abpromise guarantee covers the use of ab7398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Pre-clearing with a non-specific sheep IgG is helpful to reduce background.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.

靶标

图片

  • Western blot - Anti-Thymidylate Synthase antibody (ab7398)
    Western blot - Anti-Thymidylate Synthase antibody (ab7398)
    ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody (ab7398)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody (ab7398)
    IHC image of Thymidylate Synthase staining in Human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7398, 5µg/ml, for 15 mins at room temperature. A Rabbit anti-Sheep biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Gu YJ  et al. GRP78 confers the resistance to 5-FU by activating the c-Src/LSF/TS axis in hepatocellular carcinoma. Oncotarget 6:33658-74 (2015). WB . Read more (PubMed: 26378040) »
  • MacFarlane AJ  et al. Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity. J Nutr 144:419-24 (2014). Read more (PubMed: 24500934) »

See all 6 Publications for this product

发表研究结果有使用 ab7398？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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6 reviews or Q&A

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Thank you very much for Proforma invoice. We will inform our customer and after then we will send an official order. But Hthe customer would like to know, if POSITIVE CINTROL is possible to buy from Abcam.

Thank you for your enquiry. For ab7398 and 3145 - you can use 5-FU-resistant cancer cell lines or human breast, colorectal, gastric, head and neck carcinomas as positive controls. All the cells especially the rapidly proliferating cells express this...

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Please would you let me know the subclass of the antibody to thymidylate synthase and whether the light chain is kappa or lambda. Also, what is the sensitivity of the antibody.

This is a polyclonal antibody, and so we cannot refer to subclasses. The concentration is 10mg/ml and the sensitivity can be seen from the recommended concentrations for each application.

Is the antibody directed to the N-terminal?

This antibody was raised against the full length recombinant protien. The epitopes recognised by this antibody have not been mapped.

Would you please let me know what is the carrier protein for the antibody.

This antibody was raised against a recombinant protein. A carrier protein was not used for immunisation.

Do you have an example of the results obtained for a Western Blot for this antibody. Also the quote is for 1 mg of antibody. Is this 1 mg of an IgG fraction or for purified antibody?

The antibody is an IgG fraction. Unfortunatly we do not have an example to hand of the Western blot. However, the antibody works very well in Western blotting and we recommend this antibody for this application.

Does your antibody (THymidylate Synthase) work on paraffin?

Yes, the anti-hTS does work on paraffin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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