Anti-Thymidylate Synthase抗体(ab34836)

概述

  • 产品名称Anti-Thymidylate Synthase抗体
    参阅全部 Thymidylate Synthase 一抗
  • 描述
    兔多克隆抗体to Thymidylate Synthase
  • 特异性This antibody recognizes Thymidylate Synthase and an additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. (This event occurs in most human breast, colorectal, gastric, head and neck carcinomas.) The antibody can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
  • 经测试应用适用于: ELISA, IP, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, African Green Monkey
  • 免疫原

    Recombinant full length protein (Human) expressed in E. coli.

  • 阳性对照
    • HeLa cell extracts.

性能

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应用

Our Abpromise guarantee covers the use of ab34836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/100000.
IP 1/500.
WB 1/1000. Predicted molecular weight: 36 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

靶标

Anti-Thymidylate Synthase antibody 图像

  • ICC/IF image of ab34836 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Thymidylate Synthase antibody (ab34836)参考文献

ab34836 has not yet been referenced specifically in any publications.

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