Thymidine Kinase 1 overexpression 293T裂解液(全细胞) (ab94157)

概述

  • 产品名称Thymidine Kinase 1 overexpression 293T裂解液(全细胞)
  • 常规说明ab94157 is a 293T cell transfected lysate in which Human Thymidine Kinase 1 has been transiently over-expressed using a pCMV-Thymidine Kinase 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Similarity: Belongs to the thymidine kinase family. PTM: Phosphorylated on Ser-13 in mitosis.

应用

Our Abpromise guarantee covers the use of ab94157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Thymidine Kinase 1 overexpression 293T lysate (whole cell) 图像

  • ab94157 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Thymidine Kinase 1 antibody (ab57757) at 1/500 dilution

    Lane 1 : Thymidine Kinase 1 overexpression 293T lysate (whole cell) (ab94157)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Thymidine Kinase 1 overexpression 293T lysate (whole cell) (ab94157)参考文献

ab94157 has not yet been referenced specifically in any publications.

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