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RabMAb

Anti-Thymidine Kinase 1抗体[EPR3194] (ab91651)

概述

  • 产品名称
    Anti-Thymidine Kinase 1抗体[EPR3194]
    参阅全部 Thymidine Kinase 1 一抗
  • 描述
    兔单克隆抗体[EPR3194] to Thymidine Kinase 1
  • 经测试应用
    适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Hela cells and Molt-4 cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Thymidine Kinase 1 antibody (Phycoerythrin) [EPR3194] (ab208998)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR3194
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for IHC-P or IP.
  • 靶标

    • 序列相似性
      Belongs to the thymidine kinase family.
    • 翻译后修饰
      Phosphorylated on Ser-13 in mitosis.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • cytosolic antibody
      • KITH_HUMAN antibody
      • Thymidine kinase 1 antibody
      • Thymidine kinase 1 soluble antibody
      • Thymidine kinase 1 soluble isoform antibody
      • Thymidine kinase antibody
      • Thymidine kinase cytosolic antibody
      • TK 1 antibody
      • TK 2 antibody
      • TK1 antibody
      • Tk1a antibody
      • Tk1b antibody
      • TK2 antibody
      see all

    图片



    • Predicted band size : 25 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Jurkat whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab91651 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab91651 was shown to recognize TK1 when TK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and TK1 knockout samples were subjected to SDS-PAGE.  Ab91651 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • ab91651 at 1/100 dilution staining Thymidine kinase 1 in Hela cells
    • Anti-Thymidine Kinase 1 antibody [EPR3194] (ab91651) at 1/500 dilution + Molt-4 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 25 kDa

    文献

    ab91651 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you for your enquiry.

    I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated...

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    Thank you for contacting Abcam.

    The main difference between ab76495 and ab91651 is that they will have different epitope recognition sites.

    Also for ab76495, we guarantee it to react with mouse and human tissue in the following appl...

    Read More

    Thank you very much for your enquiry. Based on the immunogen sequences both Thymidine Kinase 1 (TK1) antibodies ab76495 and ab91651 are specific for TK1 and will not recognise TK2: The immunogen sequence for ab76495 is a peptide near the N-termin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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