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RabMAb

Anti-Thymidine Kinase 1抗体[EPR3193] (ab76495)

概述

  • 产品名称
    Anti-Thymidine Kinase 1抗体[EPR3193]
    参阅全部 Thymidine Kinase 1 一抗
  • 描述
    兔单克隆抗体[EPR3193] to Thymidine Kinase 1
  • 经测试应用
    适用于: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Thymidine Kinase 1 aa 1-100. The exact sequence is proprietary.

  • 阳性对照
    • WB: Molt-4 cell lysate; IHC-P: human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-Thymidine Kinase 1 antibody (Alexa Fluor® 488) [EPR3193] (ab201259)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR3193
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/5000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP 1/10.
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 序列相似性
    Belongs to the thymidine kinase family.
  • 翻译后修饰
    Phosphorylated on Ser-13 in mitosis.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

图片



  • Predicted band size : 25 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: MOLT4 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76495 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab76495 was shown to specifically react with TK1 when TK1 knockout samples were used. Wild-type and TK1 knockout samples were subjected to SDS-PAGE.  Ab76495 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded human tonsils using 1/100 ab76495
  • Overlay histogram showing HeLa cells stained with ab76495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76495, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-Thymidine Kinase 1 antibody [EPR3193] (ab76495) at 1/50000 dilution + Molt-4 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

文献

This product has been referenced in:
  • Marthandan S  et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . Read more (PubMed: 27140416) »
  • Zhu P  et al. Synergistic effect of a tissue kallikrein 1 and tissue inhibitor of matrix metalloproteinase 1 co-expression vector on the proliferation of rat vascular smooth muscle cells. Mol Med Rep 12:5671-8 (2015). WB . Read more (PubMed: 26252163) »

See all 7 Publications for this product

客户评价及客户问答

Thank you for your enquiry.

I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated...

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Thank you for contacting Abcam.

The main difference between ab76495 and ab91651 is that they will have different epitope recognition sites.

Also for ab76495, we guarantee it to react with mouse and human tissue in the following appl...

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Thank you very much for your enquiry. Based on the immunogen sequences both Thymidine Kinase 1 (TK1) antibodies ab76495 and ab91651 are specific for TK1 and will not recognise TK2: The immunogen sequence for ab76495 is a peptide near the N-termin...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (T cells)
Specification
T cells
Fixation
Formaldehyde
Permeabilization
Yes - SAPONIN
Gating Strategy
CD4 T cells, live
Username

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Verified customer

提交于 Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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