RabMAb

Anti-Thymidine Kinase 1抗体[EPR3193] (ab76495)

概述

  • 产品名称Anti-Thymidine Kinase 1抗体[EPR3193]
    参阅全部 Thymidine Kinase 1 一抗
  • 描述
    兔单克隆抗体[EPR3193] to Thymidine Kinase 1
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Thymidine Kinase 1 aa 1-100. The exact sequence is proprietary.

  • 阳性对照
    • WB: Molt-4 cell lysate; IHC-P: human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-Thymidine Kinase 1 antibody (Alexa Fluor® 488) [EPR3193] (ab201259)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR3193
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/5000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP 1/10.
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 序列相似性Belongs to the thymidine kinase family.
  • 翻译后修饰Phosphorylated on Ser-13 in mitosis.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

Anti-Thymidine Kinase 1 antibody [EPR3193] 图像

  • Anti-Thymidine Kinase 1 antibody [EPR3193] (ab76495) at 1/50000 dilution + Molt-4 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • Immunohistochemical analysis of paraffin-embedded human tonsils using 1/100 ab76495
  • Overlay histogram showing HeLa cells stained with ab76495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76495, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Thymidine Kinase 1 antibody [EPR3193] (ab76495)参考文献

This product has been referenced in:
  • Breitbach CJ  et al. Oncolytic vaccinia virus disrupts tumor-associated vasculature in humans. Cancer Res 73:1265-75 (2013). Read more (PubMed: 23393196) »
  • Viel T  et al. Early assessment of the efficacy of temozolomide chemotherapy in experimental glioblastoma using [18F]FLT-PET imaging. PLoS One 8:e67911 (2013). IHC-P ; Mouse . Read more (PubMed: 23861829) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry.

I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated...

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Thank you for contacting Abcam.

The main difference between ab76495 and ab91651 is that they will have different epitope recognition sites.

Also for ab76495, we guarantee it to react with mouse and human tissue in the following appl...

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Thank you very much for your enquiry. Based on the immunogen sequences both Thymidine Kinase 1 (TK1) antibodies ab76495 and ab91651 are specific for TK1 and will not recognise TK2: The immunogen sequence for ab76495 is a peptide near the N-termin...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (T cells)
Specification T cells
Fixation Formaldehyde
Permeabilization Yes - SAPONIN
Gating Strategy CD4 T cells, live
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提交于 Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"