Anti-Thymidine Kinase 1抗体(ab75277)

概述

  • 产品名称Anti-Thymidine Kinase 1抗体
    参阅全部 Thymidine Kinase 1 一抗
  • 描述
    兔多克隆抗体to Thymidine Kinase 1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 205 and 234 of human Thymidine Kinase (NP_003249.1)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab75277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 序列相似性Belongs to the thymidine kinase family.
  • 翻译后修饰Phosphorylated on Ser-13 in mitosis.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

Anti-Thymidine Kinase 1 antibody 图像

  • All lanes : Anti-Thymidine Kinase 1 antibody (ab75277) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 130 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
    ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-Thymidine Kinase 1 antibody (ab75277)参考文献

ab75277 has not yet been referenced specifically in any publications.

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