Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
ab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Use at 2-5 µg/mg of lysate.
Anti-Thymidine Kinase 1 antibody 图像
Western blot - Thymidine Kinase antibody (ab75277)
All lanes : Anti-Thymidine Kinase 1 antibody (ab75277) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 25 kDa Observed band size : 25 kDa Additional bands at : 130 kDa,65 kDa. We are unsure as to the identity of these extra bands. Exposure time : 30 seconds
Immunoprecipitation - Thymidine Kinase antibody (ab75277)
Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Anti-Thymidine Kinase 1 antibody (ab75277)参考文献
has not yet been referenced specifically in any publications.
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