Anti-Thymidine Kinase 1抗体(ab59271)


  • 产品名称Anti-Thymidine Kinase 1抗体
    参阅全部 Thymidine Kinase 1 一抗
  • 描述
    兔多克隆抗体to Thymidine Kinase 1
  • 特异性ab59271 detects endogenous levels of total Thymidine Kinase protein.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    A synthesized non-phosphopeptide derived from human Thymidine Kinase around the phosphorylation site of serine 13.

  • 阳性对照
    • Extracts from COLO205 cells; human breast carcinoma tissue.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab59271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.


Anti-Thymidine Kinase 1 antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab59271 at a dilution of 1/50. Left hand image - without immunising peptide; right hand image - with immunising peptide.
  • All lanes : Anti-Thymidine Kinase 1 antibody (ab59271) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells with no immunising peptide
    Lane 2 : extracts from COLO205 cells with immunising peptide

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

Anti-Thymidine Kinase 1 antibody (ab59271)参考文献

ab59271 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-11%)
Sample Rat Tissue lysate - whole (Lung)
Specification Lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RM°C

Abcam user community

Verified customer

提交于 Dec 13 2013

Thank you for your enquiry.

I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated...

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Thank you for contacting us.

To our knowledge this product has not been tested for cross reactivity against Thymidine kinase 2 (TK2; SwissProt ID O00142). However, as the homology of the two proteins is extremely poor at only 14%, we do not f...

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Glad to hear that the replacement vial was successfull. I checked our stocks and we have 3 more vials of ab59271, lot GR54001-1 in stock. It is the only lot in stock right now but just in case, simply request the specific lot you are interested in purc...

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Thanks for  your reply. So you would like 1 vial of ab59271 of a different lot and 2 vials of ab47681. That leaves you with 1 more antibody to replace the 4 vials of ab59271 you tested that did not work. Please let me know what add...

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Thank you for sending along the purchase order numbers. I see they are all for purchase of ab59271, lot number 578728. Have you tried each vial of antibody to determine if perhaps it is a vial-specific issue?

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HELA Cell)
Loading amount 40 µg
Specification HELA Cell
Gel Running Conditions Non-reduced Denaturing (4-20% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Jun 18 2008