概述
- 产品名称Anti-THRSP抗体
参阅全部 THRSP 一抗 - 描述兔多克隆抗体to THRSP
- 经测试应用适用于: ICC/IF, WB, IHC-Frmore details
- 种属反应性与反应: Mouse
预测可用于: Rat, Human
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human THRSP.
- 阳性对照
- This antibody gave a positive signal in Mouse Liver tissue lysate. IHC: Human breast adipose frozen tissue sections.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab97504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). | |
| IHC-Fr | Use a concentration of 1 µg/ml. |
靶标
- 功能Plays a role in the regulation of lipogenesis, especially in lactating mammary gland. Important for the biosynthesis of triglycerides with medium-length fatty acid chains. May modulate lipogenesis by interacting with MID1IP1 and preventing its interaction with ACACA (By similarity). May function as transcriptional coactivator. May modulate the transcription factor activity of THRB.
- 组织特异性Mainly expressed in tissues that synthesize triglycerides.
- 序列相似性Belongs to the SPOT14 family.
- 细胞定位Nucleus. Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 7069 Human
- Entrez Gene: 21835 Mouse
- Entrez Gene: 25357 Rat
- Omim: 601926 Human
- SwissProt: Q92748 Human
- SwissProt: Q62264 Mouse
- SwissProt: P04143 Rat
- Unigene: 591969 Human
see all -
别名
- Lpgp antibody
- LPGP1 antibody
- MGC21659 antibody
see all
Anti-THRSP antibody 图像
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Immunohistochemistry (Frozen sections) - Anti-THRSP antibody (ab97504)IHC image of THRSP staining in human breast adipose frozen tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97504, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - THRSP antibody (ab97504)Anti-THRSP antibody (ab97504) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 17 kDa
Observed band size : 17 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes -
Immunocytochemistry/ Immunofluorescence - THRSP antibody (ab97504)ICC/IF image of ab97504 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97504 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%, 5min) Hek293, HepG2, and MCF-7 cells at 1ug/ml.
Anti-THRSP antibody (ab97504)参考文献
ab97504 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"