Anti-Thromboxane synthase抗体(ab39362)

概述

  • 产品名称
    Anti-Thromboxane synthase抗体
    参阅全部 Thromboxane synthase 一抗
  • 描述
    兔多克隆抗体to Thromboxane synthase
  • 特异性
    This antibody is specific for Thromboxane synthase.
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-FoFr, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
    预测可用于: Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    TNPDCQEKLLREVDVFKEK

    , corresponding to amino acids 359/377 of Human Thromboxane synthase

  • 阳性对照
    • Whole cell lysate from platelets.

性能

应用

Our Abpromise guarantee covers the use of ab39362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).Can be blocked with Human Thromboxane synthase peptide (ab72365).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 组织特异性
    Platelets, lung, kidney, spleen, macrophages and lung fibroblasts.
  • 疾病相关
    Defects in TBXAS1 are the cause of Ghosal hematodiaphyseal dysplasia (GHDD) [MIM:231095]. GHDD is a rare autosomal recessive disorder characterized by increased bone density with predominant diaphyseal involvement and aregenerative corticosteroid-sensitive anemia. Aregenerative anemia is characterized by bone marrow failure, so that functional marrow cells are regenerated slowly or not at all.
    Defects in TBXAS1 are the cause of thromboxane synthetase deficiency (TBXAS1 deficiency) [MIM:274180]. It is characterized by hemorrhagic diathesis.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BDPLT14 antibody
    • CYP5 antibody
    • CYP5A1 antibody
    • Cytochrome P450 5A1 antibody
    • Cytochrome P450, family 5, subfamily A, polypeptide 1 antibody
    • FLJ52771 antibody
    • GHOSAL antibody
    • Platelet cytochrome P450 subfamily V antibody
    • Tbxas1 antibody
    • THAS antibody
    • THAS_HUMAN antibody
    • Thromboxane A synthase 1 (platelet) antibody
    • Thromboxane A synthase 1 (platelet, cytochrome P450, family 5, subfamily A) antibody
    • Thromboxane A synthase 1 antibody
    • Thromboxane A synthase 1 platelet cytochrome P450 subfamily V antibody
    • Thromboxane-A synthase antibody
    • TS antibody
    • TXA synthase antibody
    • TXAS antibody
    • TXS antibody
    see all

图片

  • All lanes : Anti-Thromboxane synthase antibody (ab39362) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

文献

This product has been referenced in:
  • Pai CH  et al. Lack of Thromboxane Synthase Prevents Hypertension and Fetal Growth Restriction after High Salt Treatment during Pregnancy. PLoS One 11:e0151617 (2016). IHC . Read more (PubMed: 26974824) »
  • Font-Nieves M  et al. Induction of COX-2 Enzyme and Down-regulation of COX-1 Expression by Lipopolysaccharide (LPS) Control Prostaglandin E2 Production in Astrocytes. J Biol Chem 287:6454-68 (2012). WB ; Mouse . Read more (PubMed: 22219191) »

See all 7 Publications for this product

客户评价及客户问答

Thank you for the additional information about your samples and protocol. I will be happy to issue a refund if you like. If you are interested, we also have another antibody which I recommend as a free replacement, ab68823:

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